Preparation method for nano chemical light emitting probe capable of natural degrading in living body

A technology of chemiluminescence and chemiluminescence reagents, which is applied in the field of preparation of nanoscale chemiluminescence probes, can solve the problems of unobservable reaction phenomena and difficult real-time detection, etc., and achieves simple production methods, improved detection sensitivity, and obvious experimental results Effect
CN1223847CInactive Publication Date: 2005-10-19SOUTH CHINA NORMAL UNIVERSITY
0 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SOUTH CHINA NORMAL UNIVERSITY
Publication Date
2005-10-19
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The characteristics of the chemiluminescnece probe are that the soluble protein with the grain sizes less than 150nm coats the surface of the chemiluminescence reagent. The preparing method includes following steps. (1) chemiluminescence reagent, with the vegetable oil being added into. Under 0-4 deg.C, the amixture is processed by the ultrasonic in the intensity within 80-220W, for 2-5 min, with the nonionic surfactant being added into. The admixture presents the even ivory. (2) The emulsion produced is sub-packaged. The precipitable oil caused by centrifugalizing the emulsion is removed. (3) Heating solidifies the emulsion. The solidified emulsion is conserved at the temperature lower than 4 deg.C. The invention solves the instantaneous reaction between the chemiluminescence reagent MCLA and the oxygen-derived free radicals.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to a preparation method of a nanoscale chemiluminescent probe that can be naturally degraded in a living body. Background technique

[0002] Chemiluminescent probes are mainly used to detect free radicals, and the reaction between the two is instantaneous and violent. Therefore, it is difficult for the existing chemiluminescent probes to detect the chemiluminescence of biological tissues in vivo in real time. As a result, some reaction phenomena cannot be observed, resulting in experimental artifacts. Contents of the invention

[0003] The object of the present invention is to provide a kind of preparation method of the nano-scale chemiluminescent probe that can degrade naturally in organisms, solve the problem of simple chemiluminescent reagent MCLA and oxygen free radical ( 1 o 2 , O 2 - ) is instantaneous and short-lived, it is difficult to achieve real-time long-term observation.

[0004] The nano-scale chemiluminescenc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More