Method for producing apomictic plants

A technology of asexual reproduction and sexual reproduction, applied in botany equipment and methods, horticultural methods, plant gene improvement, etc., can solve problems such as unreported expression

Inactive Publication Date: 2000-09-20
UTAH STATE UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] Two research groups are currently attempting to introgression apomixis from Tripsacum into maize, neither of them has reported its expression in additional lines containing less than nine Tripsacum chromosomes

Method used

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  • Method for producing apomictic plants
  • Method for producing apomictic plants
  • Method for producing apomictic plants

Examples

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Embodiment 1

[0068] Selection and Collection of Germplasm - Dicotyledonous Plants

[0069] It is a feature of the present invention to provide methods for selecting and collecting the most suitable strains from a species or a population of closely related species to produce vegetative plants. These approaches are thought to mimic and greatly accelerate the natural processes that initiated the evolution from sexual to asexual plants, ie, those that lead to secondary encounters among taxa adapted to widely divergent climates and photoperiods.

[0070] In this example, a preferred method for the Dicotyledonous subclass is explained in detail. In Example 2, a preferred method for the subclass of Monocots is explained in detail. Dicotyledonous examples include sexual species from the genus Antennaria (X=14). One of ordinary skill in the art is expected to be able to successfully apply this method to a number of species, including various dicot crops such as strawberries, Raphanobrassica, pota...

Embodiment 2

[0076] Selection and Collection of Germplasm - Monocots

[0077] In this example, the method for selecting and collecting germ plasm from the subclass of monocots is explained in detail. This monocot example comprises a sexual species from the genus Tripsacum (X=18). One of ordinary skill in the art is expected to be able to successfully apply this method to a number of species, including various monocotyledonous crops such as sorghum, wheat, barley, rice and corn.

[0078] This example relates to the monocot genus Tripsacum, which is endemic to the New World and lives at 42°N-24°N latitudes (de Wet et al., Systematics of Tripsacum dactyloides (Gramineae), 69 American Journal of Botany 1251-57( 1982) (incorporated herein by reference), from 0-2600 m altitude (Berthaud et al., Tripsacum: Diversity and conservation in Taba, Genetic Resources of Maize (CIMMYT, 1995) (incorporated herein by reference)). Hermaphroditism herbaceous perennial plant with male and female flowers sepa...

Embodiment 3

[0083] Quantification of the effect of different photoperiods on flowering

[0084] One of the features of the present invention is to provide a method to quantify the effect of the sexual lines selected as the most suitable for the preparation of asexual plants (Examples 1 and 2) on the development of flowers at different photoperiods. It should be appreciated that many methods for quantifying this effect have been published in the recent literature, and those skilled in the art will find that methods other than those described here are more suitable for certain species.

[0085] The currently preferred method for quantifying the photothermal response of Antennaria species that are native to temperate (mid-latitude) to alpine climates (high-latitude) was modified from the traditional study of Oxyria digyna (Mooney and Billings, Oxyria digyna Arctic Comparative Physiological Ecology of Alpine and Alpine Populations, 31 Eco Monog. 1-29 (1961) (hereby incorporated by reference))...

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Abstract

Methods are provided for producing apomictic plants from sexual plants divergent with respect to responses to different photoperiods and schedules of megaspore and gametophyte development. A preferred system is to identify divergent lines from within a species or closely related group of species, accentuate the divergence by breeding where necessary, and produce artificial amphiploids that contain genomes from the apposing divergent lines. Apomixis results from the asynchronous expression of female developmental programs induced by combining the reproductively divergent lines. The procedures for manipulating the expression of apomixis described herein permit the development of true-breeding hybrids of various cultivated crops.

Description

[0001] Cross-references to related applications [0002] This application claims the benefit of US Provisional Application 60 / 037,211, filed February 5,1997. Background of the invention [0003] The present invention relates to a process for preparing plants from normally sexually reproducing plants which can genetically clone themselves through their own seeds (gametophytic apomicts). More particularly, the present invention relates to methods comprising (a) selecting two or more sexual reproductive lines expressing reproductive phenotypes that differ from each other, which in some cases requires plant breeding and selection to obtain a sufficient degree of divergence , (b) crosses between plants with different reproductive phenotypes, and (c) diploidization (doubling of chromosomes) before or after the cross. [0004] Asexual reproduction appears to enhance the production of food, feed and fiber more than any other plant mechanism. Asexual reproduction occurs in about 0.3%...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/00A01H1/04
CPCA01H1/04A01H1/022
Inventor J·G·卡曼
Owner UTAH STATE UNIVERSITY
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