Process for rourifying insuline

Abstract

A process for purifying insuline includes such steps as salting out, sodium salt crystallizing, zinc salt recrystallizing, and collecting dried insuline crystal powder. Its advantages are short period and high output rate.

Description

technical field [0001] The present invention relates to a purification method of insulin, more specifically a crystallization method of insulin, which is used to prepare high-purity insulin. Background technique [0002] At present, most of the known insulin production adopts the zinc salt crystallization process, and the process flow is: salt solution solution → acid protein removal → filtration → zinc precipitation → dissolution → alkali protein removal → filtration → crystallization → collection and drying. Its disadvantages are low product yield, many operation steps, long production cycle, high consumption of auxiliary materials, and about 15% of recycled products are produced in the process at the same time, especially for the production of high-purity insulin. Obviously, its process is unreasonable. Chinese patent Publication No. CN1077203A discloses an invention patent application "a crystallization process of insulin sodium salt", the process of which is: salt solut...

AI Technical Summary

Problems solved by technology

Its disadvantages are low product yield, many operation steps, long production cycle, high consumption of auxiliary materials, and about 15% of recycled products are produced in the process at the same time, especially for the production of high-purity insulin. Obviously, its process is unreasonable. Chinese patent Publication No. CN1077203A discloses an invention patent application "a crystallization process of insulin sodium salt".

Compared with the zinc salt crystallization process, this process has fewer procedures and a shorter production cycle, but the disadvantage is that the titer yield is only increased by 3-5%.