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Method for prodn. of vegetable peptone by enzyme method using soyabean as raw material

A technology for plant peptone and soybean protein, which is applied in the field of peptone production, can solve the problems of low quality, high cost, complex production process, etc., and achieves the effects of high yield, low cost, simple process and equipment

Inactive Publication Date: 2003-05-21
范铁生
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its production process is complicated, the cost is high, and the quality is low, so it has not formed industrialized production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Soybeans are screened, degreased at low temperature, and crushed to a certain fineness. Then soak in hot water, the water temperature is 80°C, and the pH value is 7.6. In order to improve the solubility of soybean protein, a Nacl solution with a concentration of 0.5-2 mol / L is added to the feed liquid. After soaking for 2 hours, filter and remove the slag, and send the feed liquid into the digestion and hydrolysis tank. Adjust the feed liquid temperature to 60°C and the pH value to 7.5. Then add papain. The added amount is 4-5% of the soybean protein weight content in the feed liquid. The digestion and hydrolysis time is 3 hours. Afterwards, adjust the pH value of the feed liquid to 5.0, heat up to 100° C., and keep the temperature for 5 minutes. Then, it is filtered, concentrated and dried to obtain plant peptone powder.

Embodiment 2

[0015] After soybeans are degreased at low temperature, crushed, soaked in hot water, salted and filtered, the feed liquid is sent to the hydrolysis digester. Adjust the feed liquid temperature to 45°C and the pH value to 7.5. Then add 1398 enzyme hydrolysis digestion. The addition amount is 8% of the soybean protein weight content in the feed liquid. The digestion and hydrolysis time is 3 hours. After hydrolysis and digestion, adjust the pH value of the feed solution to 5.0, heat up to 100°C, and keep the temperature for 5 minutes. Then remove residue and filter again, concentrate and dry to obtain plant peptone powder.

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PUM

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Abstract

In the production method of plant peptone, soybean is used as raw material, which includes the following steps: low-temp. defatting soybean, pulverizing, soaking in hot water, heating and heat-insulating for a certain time, sterilizing, regulating pH value and temp. at 45 deg.C-60 deg.C, adding a certain quantity of soybean protein digestive enzyme to make hydrolysis, regulating pH value, heatingto 100 deg.C, heat-insulating for 5 min., filtering, removing residue, concentrating and drying so as to obtain the invented product powder preparation.

Description

(1) Technical field [0001] The invention relates to a method for producing peptone, in particular to a method for producing plant peptone using soybeans as raw materials. (2) Background technology [0002] Peptone is widely used as a culture medium in the fields of microbiology and pharmacy. Plant peptone, especially plant peptone using soybean as raw material, has attracted more and more attention and attention in the field of bioengineering due to its special performance, low price, and excellent economics such as easy availability of raw materials. However, soy peptone used as a reagent and industrial grade soy peptone have not yet been commercially produced in China. According to a new search by the Institute of Science and Technology Information of China, although Shanxian Biochemical Factory in Henan Province reported the achievement of producing plant peptone by enzymatic method, it did not elaborate on the main content of the technology. For example, what kind of p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J3/16A23J3/34
Inventor 范铁生
Owner 范铁生
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