Screening method of salt enduring species of tomato

A screening method and salt-tolerant technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of only obtaining salt-tolerant plants, few reports of bred varieties, and reduced screening effectiveness, so as to achieve excellent agronomic traits , Stable agronomic traits and reliable screening results

Inactive Publication Date: 2003-08-06
SHANGHAI JIAO TONG UNIV
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AI Technical Summary

Problems solved by technology

[0003] Tomato is a strictly self-pollinating plant. Long-term artificial domestication and sexual hybridization have made its genetic background narrower and narrower. Some stress-tolerant genes, such as salt-tolerant genes, are difficult to find in its cultivated species. Therefore, in recent years, domestic Many experts at home and abroad have tried to breed new tomato salt-tolerant varieties through genetic engineering technology and cell engineering technology, but most of them only have reports on salt-tolerant plants, and there are few reports on bred varieties.
The reason is that the expression of salt tolerance is mostly controlled by polygenes; the second study proves that the expression of salt tolerance in different growth stages of tomato is not the same, which brings inconvenience to the screening of salt tolerance traits, thus prolonging the Screening time is reduced, and the effectiveness of screening is reduced, which ultimately hinders the progress of salt tolerance breeding

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The total DNA of L. cheesmanii LA166, a wild relative of tomato, was introduced into the proposed improved variety “Xianfeng” by using pollen tube channeling technology, and 24 fruits were obtained in the season.

[0018] Seeds of 24 fruits were soaked for 24 hours, sterilized with saturated bleach solution for 20 minutes, and then germinated under sterile conditions, and then the germinated seeds were sown in 1 / 2 MS macronutrient medium; 10 days after emergence, The buds were excised and inoculated in MS medium supplemented with 75mmol / L NaCl. After 10 days, the rooting situation was counted, and 15 salt-tolerant plants were preliminarily determined.

[0019] The cotyledons of the above 15 plants were taken as explants, inoculated in the medium supplemented with BA2.0mg / L+IAA0.2mg / L+MS to induce callus, subcultured once every 15 days, and the subcultured 2 The second callus was inoculated in the medium with 175mmol / L NaCl+BA2.0mg / L+IAA0.2mg / L+MS added, the proliferatio...

Embodiment 2

[0025] Ten salt-tolerant plants obtained by screening somatic mutants were transplanted into seedling pots. After slowing down the seedlings, the water solution supplemented with 200mmol / L NaCl was used as the irrigation water source, and the stress screening of salt tolerance was carried out. All 10 plants showed salt tolerance. Then it was transplanted in a greenhouse, and only 2 plants grew normally and could bloom and bear fruit. Take the side branch with 4-5 leaves under the first flower spike, and treat the base of the side branch with 100mmol / L NAA for 10 minutes, then cut it into the substrate tank, and use 1 / 10MS medium supplemented with 200mmol / L NaCl as nutrition Liquid irrigation, observe and count the rooting situation of lateral branches after 10 days.

[0026] Plant the plants formed by 2 lateral branches in a greenhouse to further observe other agronomic traits of the material, and leave an ear fruit for the corresponding plant (the mother plant that takes th...

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Abstract

The method for screening tomato salt-enduring character is characterized by that it utilizes the rooting condition of NaCl forced tomato bud, multiplication condition of cotyledonary callus, growth vigor of seedling, rooting condition of collateral cutting of plant-forming stage and vigor of germination of next generation seed to comprehensively judge the sanility tolerance of tomato plant, at the same time accelerate the salt-tolerant breeding process of tomato. Said invention is simple and convenient, easy to learn, the screening process of the whole system is made under the controlled condition, so that it screening result is more reliable.

Description

Technical field: [0001] The invention relates to a screening method for salt-tolerant traits of tomato, which can be used for the cultivation of new salt-tolerant varieties of all fruits and vegetables, such as tomato, eggplant, pepper, cucumber, melon, etc. The content of side branch cuttings can be used for the cultivation of all traits, and belongs to crop breeding technology field. Background technique: [0002] With the continuous growth of the earth's population, land area and fresh water resources are becoming increasingly scarce, so research on how to improve the salt tolerance of crops is carried out all over the world. [0003] Tomato is a strictly self-pollinating plant. Long-term artificial domestication and sexual hybridization have made its genetic background narrower and narrower. Some stress-tolerant genes, such as salt-tolerant genes, are difficult to find in its cultivated species. Therefore, in recent years, domestic Many experts at home and abroad have t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陈火英庄天明刘风荣
Owner SHANGHAI JIAO TONG UNIV
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