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Method for testing bioactive substance by using electrochemistry process with oxido-reductase marked and its device

A technology of biologically active substances and reductases, which is applied in the direction of measuring devices, electrochemical variables of materials, biological testing, etc., can solve the problems of expensive testing costs of consumables, and achieve the effects of shortening the testing cycle, low testing costs, and reducing costs

Inactive Publication Date: 2003-10-08
李伟 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the problem of expensive consumables and high detection cost in the ELISA method, the present invention provides an electrochemical test method for measuring biologically active substances labeled with oxidoreductase, including:

Method used

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  • Method for testing bioactive substance by using electrochemistry process with oxido-reductase marked and its device
  • Method for testing bioactive substance by using electrochemistry process with oxido-reductase marked and its device
  • Method for testing bioactive substance by using electrochemistry process with oxido-reductase marked and its device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Wash the platinum electrode with the size of _0.4mm×1cm with distilled water and dry it, and put 4.1×10 -7 mol / L rabbit IgG dissolved in 200uL NaOH-KH pH7.6 2 Prepare the sample to be tested as an antigen-containing solution in PO4 buffer. Rabbit IgG is obtained by extracting rabbit serum, centrifuging at room temperature at 10,000 rpm for 5 minutes, and taking the supernatant. After mixing equal volumes of the antigen solution and the silk fibroin solution, coat the surface of the platinum electrode, and let it dry naturally at room temperature to form a film. Among them, silk fibroin is a viscous liquid obtained by dissolving silk in lithium bromide after being soaked and washed in 95% ethanol or absolute ethanol.

[0040] Horseradish peroxidase-labeled goat anti-rabbit IgG purchased from Huamei Bioengineering Company. Dilute with double-concentration barbiturate buffer and distilled water at a volume ratio of 2:2:1 to prepare pH8.6, C=0.05mol / l double-concentratio...

Embodiment 2

[0044] 16 platinum electrodes with a size of _0.4mm×1cm were cleaned with distilled water and dried for later use. will be 4.1×10 -7mol / L rabbit IgG dissolved in 200uL NaOH-KH pH7.6 2 Prepare the test sample containing antigen solution in PO4 buffer solution. Rabbit IgG is obtained by extracting rabbit serum, centrifuging at room temperature at 10,000 rpm for 5 minutes, and taking the supernatant. After mixing equal volumes of the antigen solution and the silk fibroin solution, coat the surface of the above eight platinum electrodes, and let it dry naturally at room temperature to form a film. Among them, silk fibroin is a viscous liquid obtained by dissolving silk in lithium bromide after being soaked and washed in 95% ethanol or absolute ethanol.

[0045] The horseradish peroxidase-labeled goat anti-rabbit IgG purchased from Huamei Bioengineering Co., Ltd. was diluted with double-concentration barbiturate buffer and distilled water at a volume ratio of 2:2:1 to prepare pH8...

Embodiment 3

[0049] 16 platinum electrodes with a size of _0.4mm×1cm were cleaned with distilled water and dried for later use. will be 2.7×10 4 / ml Salmonella dissolved in 200uL NaOH-KH pH7.6 2 Prepare the test sample containing antigen solution in PO4 buffer solution, mix the sample solution and silk fibroin solution in equal volumes, coat the surface of the above-mentioned 8 platinum electrodes, and let it dry naturally at room temperature to form a film. Among them, silk fibroin is a viscous liquid obtained by dissolving silk in lithium bromide after being soaked and washed in 95% ethanol or absolute ethanol.

[0050] Dilute the horseradish peroxidase-labeled Salmonella antibody provided by the Institut Pasteur of France with double-concentration barbiturate buffer and distilled water at a volume ratio of 2:2:1 to prepare pH8.6, C=0.05 The mol / l double-concentration barbiturate buffer was used as the electrophoresis solution, and the 8 platinum electrodes immobilized with the antigen...

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Abstract

A detection method includes fixing a biological active subject as antigen to be tested possible contained in a sample an electrode surface, carrying out idiosyncray identification combination of corresponded antibody to the subject and biological active subject in the sample to be tested fixed on the electrode, clearing away the said antibody of non-idiosyncrasy combination to get the working electrode, inserting the test electrode set composed of the working electrode and reference electrode into the catalitic material corresponding to the redox enzyme to test signals generated from reaction between marked enzyme and catalitic material in the reaction tank to further decide the exist of being tested active subjects.

Description

technical field [0001] The present invention relates to a method for electrochemically measuring biologically active substances using oxidoreductase labeling, in particular to a method for quickly combining an antibody labeled with oxidoreductase with an antigen as the biologically active substance to be detected on an electrode A method for performing electrochemical detection and a detection device thereof. Background technique [0002] The double-antibody (original) sandwich method in the commonly used enzyme-linked immunosorbent assay (ELISA) is a common method for detecting antigen (body). Enzyme-labeled antibody detects the antigen in the solution, but the consumables used in this method are expensive, the detection cost is high, and the separation and identification test cycle of biologically active substances is long. Contents of the invention [0003] In order to solve the problem of expensive consum...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/327G01N27/447G01N33/53G01N33/535G01N33/561
Inventor 李伟陈石燕潘宁李幼荣黄文尉刘凯徐炳文
Owner 李伟