Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Production method of transgenic potato for expressing infectious bronchitis virus fibre mutein

A technology for bronchitis and spike protein, applied in the field of transgenic potato production, can solve the problems of high preparation cost, strong virulence, local pain, etc., and achieve the effects of reducing production cost, short regeneration period and high regeneration system

Inactive Publication Date: 2003-12-10
ZHEJIANG UNIV
View PDF0 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] 1) Safety issues The attenuated live vaccine has the risk of immune insufficiency, exogenous or vertically transmitted pathogen contamination of the vaccine, and the risk of strong virulence after vaccination of animals
[0005] 2) Inactivated vaccines are expensive. Inactivated vaccines require a large amount of antigens and high production costs, resulting in high prices for a long time
[0006] 3) The vaccination of inactivated vaccines is cumbersome, requires injection, and causes local pain and other discomforts, etc.
[0015] (2) Pathogenic proteins can complete post-translational processing in plants, such as glycosylation and spatial folding of advanced structures, so that these recombinant protein polypeptides have the same immunogenicity as natural proteins, which is unmatched by prokaryotic expression systems , which cannot glycosylate proteins of eukaryotic origin

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Production method of transgenic potato for expressing infectious bronchitis virus fibre mutein
  • Production method of transgenic potato for expressing infectious bronchitis virus fibre mutein
  • Production method of transgenic potato for expressing infectious bronchitis virus fibre mutein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] 1. Construction of Infectious Bronchitis Virus S1 Protein Antigen Plant Expression Vector pBIibv / S1

[0052] Such as figure 1 As shown, using the infectious bronchitis virus ZJ971 strain nucleic acid as a template, according to the known cDNA nucleic acid sequence of infectious bronchitis virus [26] Specific primers were synthesized, and the S1 gene of infectious bronchitis virus was amplified by polymerase chain reaction (PCR). Because Xba I and BamH I sites were introduced into the 5' end and 3' end primers respectively, the amplified fragment was directly inserted into the corresponding site in the plasmid pBlueScriptSK(+) after being digested with Xba I and BamH I. within the site. The plasmid pBSIBV / S1 was obtained, and then sequenced through nucleotides, the sequence of which is shown in the text.

[0053] Plasmid pBS IBV / S1 contains the S1 protein antigen gene of infectious bronchitis virus, the S1 gene can be excised with restriction endonucleases Xba I and B...

Embodiment 2

[0095] 1. Infectious bronchitis virus spike protein antigen plant expression vector pBIibv / S is constructed as shown in the figure, with infectious bronchitis virus (H52 strain) nucleic acid as template, according to known infectious bronchitis virus cDNA nucleic acid sequence (26) Specific primers were synthesized, and the S protein gene of infectious bronchitis virus was amplified by polymerase chain reaction (PCR). Because Xba I and BamH I sites were introduced into the 5' end and 3' end primers respectively, the amplified fragment was directly inserted into the corresponding site in the plasmid pBlueScriptSK(+) after being digested with Xba I and BamH I. within the site. The plasmid pBS IBV / S was obtained, and then subjected to nucleotide sequencing, the sequence of which is shown in the text.

[0096] The plasmid pBS IBV / S contains the S protein antigen gene of infectious bronchitis virus respectively, and the S gene can be excised by restriction endonuclease Xba I and B...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The production method of transgenic potato for expressing infectious bronchitis virus fibromutein includes the following steps: inserting the infections bronchitis virus fibromutein gene onto the plant expression victor pB1121, using triparental crossing method to introduce the recombinant expression victor into agrobacterium, using exogenous gene to transform the part of potato tuber by means of agrobacterium mediatization method, and utilizing antibiotic resistance of regenerated plant to screen and make molecular analysis by using PCR, Southern bolt and Northern bolt to obtain the transgenic plant expressing infectious bronchitis virus fibromutein. The transgenic plant can be used for feeding chicken or the infectious bronchitis virus fibromutein extracted and purified from said transgenic plant can be made into injection to immunize chichen.

Description

technical field [0001] The invention relates to a method for producing a transgenic potato expressing the spike protein of infectious bronchitis virus. Background technique [0002] Chicken infectious bronchitis (Avian Infectious Bronchitis, English abbreviation IB) has the characteristics of acute and highly contagious infection, and is one of the most serious infectious diseases that endanger the global poultry industry. English abbreviation IBV) cause. Although conventional vaccines have played an important role in controlling the destructive epidemic of diseases, the scope of vaccination is getting wider and wider, the density of epidemic prevention is getting higher and higher (above 90%), and the amount of immunization is increasing continuously. The disease is fundamentally controlled. However, the epidemic form of IB has changed, from large-scale occurrence to regional sporadic, and the lesion type has also changed from typical or single...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/00A01H4/00A01H5/00C12N1/21C12N5/10C12N15/50C12N15/74C12N15/82C12Q1/68
Inventor 周继勇吴建祥程丽琴
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com