Fungus preparation for pest control of crops and its preparing method
A technology for diseases and insect pests and crops, which is applied in the field of bacterial agents for the control of crop diseases and insect pests and its preparation. It can solve the problems that bacteria and pests are prone to drug resistance, are easily attacked by bacteria and pests, and affect the yield and quality of crops, so as to prevent reproduction. and invade the host, low processing cost, stable effect
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[0019] specific implementation plan
[0020] Such as figure 1 Shown preparation method embodiment 1 of the present invention, what it produces is the suspension dosage form of bacterial agent of the present invention, and its preparation steps are:
[0021] Step (1) The screened and rejuvenated Bacillus subtilis strains and Bacillus thuringiensis strains were made into sand tube strains according to the traditional method, and stored in a -4°C refrigerator for later use.
[0022] Step (2) pick the bacillus subtilis bacterial classification into the test tube and cultivate on the medium on the surface of the test tube, then transfer to the surface of the Kirschner flask to cultivate; put 150ml of the liquid medium in the 500ml Erlenmeyer flask, and insert it into the medium after autoclaving The surface strains are placed in a rotating speed of 220 rev / min shake flasks for cultivation, and the above-mentioned cultivation time is 3 to 4 days; the strains are placed on the ferme...
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