Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing colloidal nano granules of selenium

A technology of colloidal selenium and particles, applied in the direction of element selenium/tellurium, measuring devices, instruments, etc., can solve the problems of low yield, long time, low sensitivity of immunochromatography, etc., and achieve high yield, enhanced stability, and high sensitivity Effect

Inactive Publication Date: 2004-03-10
FIELD OPERATION BLOOD TRANSFUSION INST OF PLA SCI ACAD OF MILITARY
View PDF2 Cites 18 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The raw material used in the preparation of colloidal selenium particles abroad is SeO 2 , needs to be carried out under heating conditions, the reaction needs about 1 hour, the time is long, the OD520 reaches 1.457, the yield is low (US5348891), the sensitivity of the marker used for immunochromatography is low, and the detection of hepatitis B surface antigen (HBsAg) The lower limit of detection is 2.5ng / mL

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0018] 3. Product handling: The product can be stored at 4°C for 2 months. Example 2 Detection of HBsAg by Immunochromatography

[0019] 1. Antibody labeling

[0020] 1.1 Reagent 0.2mol / L K 2 CO 3 , 10% PEG20000, 10% BSA, TBS preservation solution (0.02mol / L Tris, 0.15mmol / L NaCl, 1% BSA, 0.05% NaN 3 )

[0021] 1.2 Treatment of colloidal selenium

[0022] 1.2.1 Centrifuge at 1000r / min for 1 minute to remove large particles.

[0023] 1.2.2 Adjust the OD value of colloidal selenium to 15.0 with deionized water.

[0024] 1.2.3 With 0.2mol / L K 2 CO 3 Adjust colloidal selenium pH=7.5.

[0025] 1.3 Antibody labeling

[0026] 1.3.1 Add 5-20 μg of antibody to be labeled into 1ml of colloidal selenium solution, mix well, and label for 10 minutes.

[0027] 1.3.2 Add 10% BSA to a final concentration of 1%, mix well and let stand for 10 minutes.

[0028] 1.3.3 Centrifuge at 7000r / min for 5 minutes, wash the supernatant and wash once with TBS solution.

[0029] 1.3.4 Resuspend...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A colloidal Se nanoparticle is prepared from seleninic acid and ascorbic acid at ordinary temp. It can be used as the marker in immunochromatography with high sensitivity and speed. Its advantages are short preparing period and high output.

Description

technical field [0001] The invention relates to a method for preparing colloidal nano selenium particles at normal temperature. Background technique [0002] Rapid detection is needed in many situations, such as emergency department, on-site mass screening, on-site blood test and home self-examination, etc. Among the many existing rapid detection methods, immunochromatography is the most highly sensitive, specific and requires the shortest time. Colloidal gold-labeled immunochromatographic detection method is more commonly used. The key technologies of this method include the synthesis of colloidal particles, protein labeling and the manufacturing and processing technology of chromatography devices. Colloidal gold particles can be prepared by the reduction reaction of sodium citrate to gold chloride, and the size of colloidal gold particles can be changed by adjusting the concentration of sodium citrate. Abroad also useful colloidal gold particles and colloidal selenium pa...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C01B19/02G01N33/532
Inventor 刘晓达李保昌王全立彭剑淳周锡鹏孙萍杨淑华
Owner FIELD OPERATION BLOOD TRANSFUSION INST OF PLA SCI ACAD OF MILITARY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com