Method for preparing colloidal nano granules of selenium

A technology of colloidal selenium and particles, applied in the direction of element selenium/tellurium, measuring devices, instruments, etc., can solve the problems of low yield, long time, low sensitivity of immunochromatography, etc., and achieve high yield, enhanced stability, and high sensitivity Effect

Inactive Publication Date: 2004-03-10
FIELD OPERATION BLOOD TRANSFUSION INST OF PLA SCI ACAD OF MILITARY
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  • Abstract
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Problems solved by technology

The raw material used in the preparation of colloidal selenium particles abroad is SeO 2 , needs to be carried out under heating conditions, the reaction needs about 1 hour, the time is long, the OD520 reac

Method used

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Examples

Experimental program
Comparison scheme
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Embodiment 2

[0018] 3. Product handling: The product can be stored at 4°C for 2 months. Example 2 Detection of HBsAg by Immunochromatography

[0019] 1. Antibody labeling

[0020] 1.1 Reagent 0.2mol / L K 2 CO 3 , 10% PEG20000, 10% BSA, TBS preservation solution (0.02mol / L Tris, 0.15mmol / L NaCl, 1% BSA, 0.05% NaN 3 )

[0021] 1.2 Treatment of colloidal selenium

[0022] 1.2.1 Centrifuge at 1000r / min for 1 minute to remove large particles.

[0023] 1.2.2 Adjust the OD value of colloidal selenium to 15.0 with deionized water.

[0024] 1.2.3 With 0.2mol / L K 2 CO 3 Adjust colloidal selenium pH=7.5.

[0025] 1.3 Antibody labeling

[0026] 1.3.1 Add 5-20 μg of antibody to be labeled into 1ml of colloidal selenium solution, mix well, and label for 10 minutes.

[0027] 1.3.2 Add 10% BSA to a final concentration of 1%, mix well and let stand for 10 minutes.

[0028] 1.3.3 Centrifuge at 7000r / min for 5 minutes, wash the supernatant and wash once with TBS solution.

[0029] 1.3.4 Resuspend...

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Abstract

A colloidal Se nanoparticle is prepared from seleninic acid and ascorbic acid at ordinary temp. It can be used as the marker in immunochromatography with high sensitivity and speed. Its advantages are short preparing period and high output.

Description

technical field [0001] The invention relates to a method for preparing colloidal nano selenium particles at normal temperature. Background technique [0002] Rapid detection is needed in many situations, such as emergency department, on-site mass screening, on-site blood test and home self-examination, etc. Among the many existing rapid detection methods, immunochromatography is the most highly sensitive, specific and requires the shortest time. Colloidal gold-labeled immunochromatographic detection method is more commonly used. The key technologies of this method include the synthesis of colloidal particles, protein labeling and the manufacturing and processing technology of chromatography devices. Colloidal gold particles can be prepared by the reduction reaction of sodium citrate to gold chloride, and the size of colloidal gold particles can be changed by adjusting the concentration of sodium citrate. Abroad also useful colloidal gold particles and colloidal selenium pa...

Claims

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Application Information

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IPC IPC(8): C01B19/02G01N33/532
Inventor 刘晓达李保昌王全立彭剑淳周锡鹏孙萍杨淑华
Owner FIELD OPERATION BLOOD TRANSFUSION INST OF PLA SCI ACAD OF MILITARY
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