Biological sensor and testing method therewith

A technology of a biosensor and a measurement method, which is applied in the field of biosensors and the measurement using the biosensor, can solve the problems of inability to perform simultaneous measurement, inability to measure, and decrease in measurement accuracy.

Inactive Publication Date: 2004-04-21
PANASONIC CORP
View PDF2 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the optimal pH of each enzyme and the type of buffer suitable for a plurality of specific substances to be measured are different, simultaneous measurement is impossible
Moreover, the scale of the device is large, and maintenance is also troublesome.
[0006] In addition, the above-mentioned conventional biosensor that continuously measures a single specific substance from a plurality of sample solutions needs to use a large-scale device such as a sample collector to continuously supply the sample solution.
Furthermore, since the measurement cannot be performed at the same time, the sample solution evaporates over time, and there is a problem that the measurement accuracy may decrease.
[0007] Moreover, in the aforementioned biosensor in which a plurality of sets of electrode systems are provided on a single substrate, if there is no time difference in the enzyme reaction times of the enzymes or combinations of enzymes contained in various reaction layers, it is impossible to separate and detect the effects of the multiple sets of electrode systems. current value
Therefore, when there is no time difference between the enzyme reaction times of various enzymes corresponding to a plurality of specific substances to be measured or when the specific substance to be measured is a single substance, the measurement cannot be performed.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Biological sensor and testing method therewith
  • Biological sensor and testing method therewith
  • Biological sensor and testing method therewith

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0063] figure 1 It is a top view of the biosensor according to Embodiment 1 of the present invention, with the separator and cover removed. figure 2 It is an exploded perspective view of the same biosensor without the reagent unit and the control unit. image 3 is the edge indicating the formation process of the electrode system part figure 1 Sectional view of the III-III line cut. Figure 4 is along figure 1 Cross-sectional view of a III-III line cut biosensor comprising a reagent part and a control part.

[0064] The production method of the biosensor 10 of this embodiment is as follows. First, a silver paste is applied by screen printing on a substrate 2 made of an insulating resin to form a measuring pole terminal 3 and a measuring pole lead wire 5 ( image 3 (b)). Next, apply a conductive carbon paste containing a resin binder to form the measuring electrode 7 ( image 3 (c)). Then apply insulating paste to form insulating layer 9 ( image 3 (d)). Apply silver ...

Embodiment approach 2

[0079] Figure 5 It is a top view of the biosensor according to Embodiment 2 of the present invention, with the separator and cover removed. Figure 6 It is an exploded perspective view of the same biosensor without the reagent unit and the control unit. Figure 7 Indicates the formation process of the electrode system part Figure 5 Sectional view of line VII-VII. Figure 8 is along Figure 5 Cross-sectional view of a biosensor comprising a reagent part and a control part cut by line VII-VII.

[0080] The production method of the biosensor 20 of this embodiment is as follows. First, on the substrate 22 made of insulating resin, silver paste is applied by screen printing method to form the terminal 23 for the measuring pole, the terminal 24 for the matching pole, the lead wire 25 for the measuring pole and the lead wire 26 for the matching pole ( Figure 7 (a)). Next, apply a conductive carbon paste containing a resin binder to form the measuring electrode 27 ( Figure ...

Embodiment approach 3

[0090] Figure 13 It is a plan view of the biosensor according to Embodiment 3, with the separator and cover removed. Figure 14 It is an exploded perspective view of the same biosensor without the reagent unit and the control unit.

[0091] The same as the manufacturing method of Embodiment 1, the substrate 52 of the biosensor 50 of this embodiment is formed with terminals 53 for measuring electrodes, lead wires 55 for measuring electrodes, measuring electrodes 57, insulating layer 59, terminals 54 for matching electrodes, and electrodes for matching electrodes. With wire 56 and matching pole 58. Next, a reagent portion is formed on the electrode system. The partition plate 62 used in the present embodiment has slits 65a, 65b, 65c, and 65d of different lengths for forming the sample solution supply channels of the respective sensor units, and these slits are connected to form the sample solution supply channel indicated by 64. An open portion of the mouth. The spacer 62 a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

A biosensor capable of measuring the concentration of one or more specific substances in one or more sample solutions almost simultaneously is provided. The biosensor comprises a plurality of sensor units, and each of the sensor units comprises an electrode system including a working electrode and a counter electrode on an insulating base plate and a reagent system including an oxidoreductase and an electron mediator. The biosensor is so configured that sample solutions supplied to the respective sensor units reach the respective reagent systems at different times. Specifically, each of the sensor units has a controlling system between a sample supply inlet and the reagent system, and the controlling system controls the time it takes for the sample solution to reach the reagent system from the sample supply inlet.

Description

technical field [0001] The present invention relates to a biosensor for measuring the concentration of a specific substance that can be contained in a sample solution and a measurement method using the biosensor. Background technique [0002] Conventionally, as a biosensor for simultaneously measuring the concentration of a plurality of specific substances, there is a flow injection type biosensor. As an example, in a biosensor using an immobilized enzyme membrane and an electrode reaction, the operation of simultaneous measurement of glucose and L-lactic acid by a liquid injection analysis device (FIA) YSI MODEL 2700 SELECT (Yellow Spring Instrument Co., Inc) was carried out. illustrate. First, the tip of the sample solution pipette is dipped into the sample solution to draw the sample solution into the measurement system, and then the sample solution is supplied to the sample solution box serving as the measurement site. At this time, a certain amount of buffer solution ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/00
CPCC12Q1/004G01N27/3272
Inventor 宫下万里子吉冈俊彦
Owner PANASONIC CORP
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap