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Method and kit for testing resistivity of insects to pesticides

A kit, insect technology, applied in biological testing, testing pharmaceutical preparations, material analysis by observing the effect on chemical indicators, etc., can solve the problem that users are not easy to use, have short shelf life, and do not know the level of insect vector susceptibility. and other problems, to achieve the effect of less manpower, long shelf life, and reduced mortality

Inactive Publication Date: 2004-06-09
MEDICAL RES INST
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Problems solved by technology

However, the continued use of insecticides has led to the development of resistance to vectors carrying the above diseases, mainly insects, which reduce the effectiveness of control of the vectors and the diseases they transmit
The determination of resistance is mainly based on the standard test methods of the World Health Organization (WHO), which are particularly difficult for users to use, the use of kits has a short shelf life and requires skilled personnel to perform the test and interpret the test results
As a result, the level of susceptibility of many insect vectors, which affects control outcomes, is generally unknown

Method used

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Examples

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Embodiment Construction

[0028] Prepare 5 vials containing the following: 10 ml sodium acetate buffer (pH 5), 2.5 ml anhydrous methanol, 5 mg tetramethylbenzidine, 7.5 ml sodium acetate buffer (pH 5) and 5 ml hydrogen peroxide ( 3%).

[0029] For the test, anhydrous methanol was poured into tetramethyl-benzidine and shaken to completely dissolve the tetramethyl-benzidine, and the resulting solution was buffered with 7.5 ml of sodium acetate buffer. Homogenize one mosquito in a drop of buffer in an Eppendorf tube with a pestle, then dilute with buffer to the 1 ml mark of the Eppendorf tube.

[0030] Two drops of the homogenate were dropped into each well of the micro-assay plate, followed by 4 drops of tetramethyl-p-benzidine solution and one drop of hydrogen peroxide.

[0031] The results were then scored visually, checking the color by comparison to a color chart standard or by scanning at 630nm with an immunoassay reader.

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Abstract

A method for checking for elevated activity of mixed-function oxidases using a single insect, which comprises homogenizing a single insect in an acid pH buffer, preferably by grinding it in a small tube, such as an Eppendorf tube, with a pestle, adding a buffered tetramethylbenzidine solution as substrate, adding hydrogen peroxide as oxidant, and checking the blue coloration, the intensity of which indicates the degree of elevated mixed function oxidase activity. The invention also provides a test kit for use in the method, which comprises separate containers of: I. an acid pH buffer, preferably pH4 buffer II. tetramethylbenzidine III. a solvent for tetramethylbenzidine, preferably methanol, and IV. hydrogen peroxide.

Description

technical field [0001] The present invention relates to a method and a kit for measuring insect resistance to insecticides, and more particularly provides a method and kit for field use for measuring insect resistance to pyrethroid insecticides. Background technique [0002] Chemical insecticides are still currently the most important agents in the control of vector-borne diseases such as malaria, dengue fever, filariasis and others. Since the invention of the first synthetic insecticide, DDT, in 1940, the spread of these diseases and the untold number of deaths has been greatly reduced. However, the continued use of insecticides has led to the development of resistance in vectors carrying the above-mentioned diseases, mainly insects, which reduces the effectiveness of control of the vectors and the diseases they transmit. The determination of resistance is mainly based on the standard test methods of the World Health Organization (WHO), which are particularly difficult for...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/15C12Q1/26C12Q1/28
CPCG01N2333/43552C12Q1/28
Inventor 李·汉·林纳兹尼·瓦西·艾哈迈德
Owner MEDICAL RES INST
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