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Protein and virus quick-speed concentration method

A protein and virus technology, applied in the field of rapid virus enrichment and protein field, can solve the problems of long culture cycle, false negative, extraction of protein and viral RNA, etc. Effect

Inactive Publication Date: 2004-08-25
HANGZHOU JINYUAN BIOTECH
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AI Technical Summary

Problems solved by technology

The current test methods have certain limitations on the size of the sample. Proteins and viral RNA cannot be extracted from large-volume samples, which often leads to false negative test results.
Chinese patent document CN1285878 discloses a "method and device for enriching and searching for microbial samples". In this method, the enrichment culture of microorganisms is completed in a syringe or equivalent. Due to the long period of conventional culture, it is not suitable for The need for rapid testing
Rapid concentration and partial purification of proteins and virus particles have not been publicly reported in Chinese medical literature and Chinese patent literature

Method used

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Embodiment Construction

[0019] The technical solutions of the present invention are further described in detail below through the examples.

[0020] 1. Preparation of enrichment experiments:

[0021] To enrich proteins and viral particles from all samples, prepare as follows:

[0022] 1. A waste tank containing disinfectant.

[0023] 2. Straws.

[0024] 3. Pre-cool the phase-separating solvent at 4°C.

[0025] 4. 50% ethanol, pre-cooled at 4°C.

[0026] 5. Pre-cool the centrifuge to 4°C.

[0027] Prepare the following preparations for enrichment of proteins and virus particles from various samples:

[0028] 1. Mouthwash, culture supernatant, urine: 50ml centrifuge tube.

[0029] 2. Serum: 15ml centrifuge tube.

[0030] 3. Feces: 50ml centrifuge tube; saline or PBS.

[0031] 4. expectoration: 50ml centrifuge tube; physiological saline or PBS, add acetylcysteine ​​to the final concentration of 1% before use.

[0032] 5. Nasal swab: 15ml centrifuge tube; saline or PBS.

[0033] 6. Throat swab:...

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Abstract

The invention relates to a protein and virus quickly-enriching method, composed of the steps: 1), flocculating protein: diluting the sample to release protein and virus particles, and adding in agglomerant to make protein and virus particles form agglutinating matter; 2), making separate-phase deposition: adding in phase-separating agent to deposit protein and virus particles and cells between phases; 3), making secondary deposition: adding in depositing solvent to deposit them to the tube bottom; 4), eliminating clear solution and retaining deposit: eliminating top clear solution, washing, centrifuging, re-eliminating top clear solution, re-centrifuging, and then absorbing all the top clear solution to get the enriched solution. It can be finished within 10-20 minutes. It can concentrate by about 100 times, making detection rate and accuracy of protein and virus raised by 100-1000 times. Its enriching speed is specially fast.

Description

technical field [0001] The present invention relates to a protein and virus rapid enrichment method, which is suitable for extracting from various human or animal samples (including mouthwash, culture supernatant, urine, fecal extract, serum, expectoration extract, nasal swabs, etc.) Methods for rapid enrichment and partial purification of proteins and virus particles in other animal and plant samples. Background technique [0002] In the samples required for the assay, the amount of proteins and viruses varies greatly. For example, the amount of virus in clinical samples such as urine, feces, serum, etc. of patients with different disease courses and patients in the incubation period due to the virus is very small. The current testing methods have certain limitations on the sample size, and cannot extract proteins and viral RNAs from large-volume samples, which often leads to false negative test results. Chinese patent document CN1285878 discloses a "method and device for...

Claims

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Application Information

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IPC IPC(8): C12Q1/24G01N1/40
Inventor 余伟明
Owner HANGZHOU JINYUAN BIOTECH
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