Bacterial strain for preventing and treating cotton verticillium wilt and its preparing method

Abstract

The invention refers to a kind of strain for curing cotton verticillium wilt and the producing method for the fungus agent, the fungus strain is Bacillus subtilis NCD-2 strain CGMCC1019. The method uses three-level yeasting process, the inoculated strains is bacillus subtilis NCD-2; the temperature of liquid yeasting is controlled in 28-37deg.C, the initiative pH value is controlled between 7.0-8.0. on base of strain liquid yeasting, it compounds several kinds of additives and produces into agent, or condensates the yeasted liquid and gets the gemma of a fungus, then they are blended with solid fillers according to proportion 1:1000, then they are produced into solid formulation through drying and crushing. The method can be used to cure the verticillium wilt of cotton, the effect is prominent, the effect can reach 56.3-92.6%, and it has no contamination, harm, its cost is low, and it is convenient to be wide popularized.

Description

technical field [0001] The invention relates to a bacterial strain for preventing and controlling cotton verticillium wilt and a preparation method thereof, and belongs to the technical field of using microorganisms to prevent and control soil-borne plant diseases. Background technique [0002] Cotton verticillium wilt is the most important worldwide intractable disease of cotton caused by Verticillium dahliae Kleb. There is still no effective control measure. Statistics show that the incidence area of ​​cotton verticillium wilt accounts for about 50% of the sown area every year in my country, and the annual loss is nearly 2 million tons, equivalent to 1 billion yuan, which seriously affects the development of cotton production and textile industry. Therefore, controlling the harm of Verticillium wilt of cotton has become an urgent problem to be solved in production. [0003] Verticillium dahliae is a typical soil habitant fungus, and its control is ex...

AI Technical Summary

Problems solved by technology

The main reason why this fungus is so rampant and difficult to control is that it can survive for more than ten years on crop residues, or even directly in the soil, and the amount of bacteria can accumulate all year round. The current control methods, Including the application of disease-resistant varieties, chemical agents and agricultural measures, all of which cannot achieve the ideal disease prevention effect

Because of the lack of antigenic breeding materials, the selection of cotton varieties resistant to Verticillium wilt is limited; the control effect of chemical agents is not good, and the second is polluting the environment and destroying the ecological balance of farmland; the more effective agricultural measures are multi-year crop rotation, but in China In a specific agricultural environment, it is difficult to popularize and apply due to a large number of people and little land and a single crop type

[0004] The use of microorganisms to control plant diseases, especially soil-borne plant diseases, is one of the most effective control measures. At present, after searching, no reports have been found on the use of Bacillus subtilis (B.subtilis) to control cotton verticillium wilt