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Method for detecting PCR-RFLP of pig bone morphogenetic protein 15 gene polymorphism

A protein and pig bone technology, applied in genetic engineering, plant genetic improvement, botanical equipment and methods, etc., can solve problems such as infertility and early follicle formation blockage

Inactive Publication Date: 2004-10-20
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

GDF9 was directly involved in the growth and differentiation of granulosa cells, as well as the meiosis ability of oocytes, using GDF9-knockout mice. GDF9-deficient female mice lead to early follicle production being blocked, resulting in infertility

Method used

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  • Method for detecting PCR-RFLP of pig bone morphogenetic protein 15 gene polymorphism
  • Method for detecting PCR-RFLP of pig bone morphogenetic protein 15 gene polymorphism

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1 Sequencing of DNA in the coding region of BMP15 gene

[0057] The Chinese local breed Meishan pig (Chinese pig pedigree) and the foreign breed Large White pig (European pig pedigree) were selected as typical test materials, and 5 pairs of primers were designed according to the DNA sequences of the pig and human BMP15 genes to amplify exon 1 and exon 1 of the BMP15 gene. Sub 2 area.

[0058] F1-1: 5'-CTGCCTTTCACTGTTTCCTG-3'

[0059] R1-1: 5'-CTCATTTTTCTCCCCTCCAG-3'

[0060] F2-1: 5'-ACATCACAGCTCACAGCAAC-3'

[0061] R2-1: 5'-ACATGAAGCGGAGTCGTAGA-3'

[0062] F2-2: 5'-GTAGCGTCTGCCACCTACAT-3'

[0063] R2-2: 5'-CCGGAACTCAAAGAATCTCAC-3'

[0064] F2-3: 5'-AGAGCCACTGTGGTTTATCG-3'

[0065] R2-3: 5'-GAAAGGATGGAGGGAACACT-3'

[0066] F2-4: 5'-TAACCAGTGTTCCTCTCATC-3'

[0067] R2-4: 5'-CCTGGGAAACCTGATCTAGC-3'

[0068] Polymerase chain reaction (PCR) was carried out in the environment containing 50ng genomic DNA, 15mmol / L Tris-HCl, 50mmol / L KCl (pH8.0), 2mmol / L MgCl ...

Embodiment 2

[0069] Example 2 PCR-RFLP detection technology of BMP15 gene SNP

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Abstract

A bone morphogenetic protein 15 (BMP 15) gene and the PCR-RFLP method for detecting its single nucleotide polymorphism (SNP) are disclosed. Its steps include extracting hog blood genom DNA, designing primer PCR amplifying, direct sequencing to amplified product, comparing and analyzing the sequence, and detecting SNP. It provides new marker for the marker aided breeding of pig.

Description

technical field [0001] The invention belongs to the technical field of molecular biology of pigs, and in particular the invention relates to a PCR-RFLP detection technology for polymorphism of pig bone morphogenetic protein 15 gene. technical background [0002] Litter size is one of the most economically important traits in pig production, and increasing weaned piglets per sow will increase the economic return to the pig producer with minimal additional investment (Rothschild, 1996). In general, litter size depends on the breed (genetics), and litter size (from 2 to 20 piglets, with an average of 9 to 10 piglets) and embryo survival (15-40%) vary greatly between breeds . The phenotype standard deviation of litter size ranged from 2.5 to 3 piglets, and the heritability ranged from 0.10 to 0.15 (Johnson et al., Responses in ovulation rate, embryonal survival, and litter traits in swine to 14 generations of selection to increase litter size. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C12P19/34C12Q1/68
Inventor 蒋思文侯振平彭健熊远著郑嵘
Owner HUAZHONG AGRI UNIV
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