Method for estimating capability of edge-water encroachment of microbe
A technology for microbial oil flooding and microorganisms, which is applied in earth-moving drilling, fluid production, wellbore/well components, etc. Such problems as screening errors, etc., to achieve the effect of reliable evaluation
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[0021] Using artificial heterogeneous core, the water phase permeability is 0.6μm 2 , the displacement water is sterilized injection water from an oil field, and the nutrient solution is prepared from displacement water (ingredient: NH 4 Cl: 0.3%, K 2 HPO 4 : 0.1%, yeast extract 0.05%, wt%), the displacement speed is 5m / d.
[0022] The core was saturated with water, crude oil was displaced to irreducible water saturation, injected water was displaced to residual oil saturation, and then transferred to Bacillus subtilis (Makkar RS, Cameotra S S. Production of biosurfactant atmesophilic and thermophilic conditions by a strain of Bacillus subtilis. J.Ind.Microbiol.Biotechnol., 20 (1), 48-52, 1998, Bacillus subtilis B.subtilis) fermented liquid (dilute to concentration 5% with nutrient solution) 1PV (PV, the pore volume of test rock core), Seal both ends of the core and let it stand for 5 days at the experimental temperature of 37°C.
[0023] According to the prior art method,...
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