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Polysaccharide, preparation and use thereof

A polysaccharide and glucose technology, applied in the field of polysaccharides, can solve problems such as limitations and insufficient attention to polysaccharides

Inactive Publication Date: 2004-11-17
CHINA PHARM UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research on polysaccharides of terrestrial organisms (animals, plants, microorganisms, etc.) started earlier, and a variety of polysaccharides have been isolated and purified from these organisms, and their structures and functions have been studied extensively, such as Fungal polysaccharides such as Lentinan, Poria polysaccharide, Yunzhi polysaccharide and Schizophyllan which have been used clinically at present are all derived from terrestrial organisms, while the research on polysaccharides from marine organisms is mostly limited to the marine organisms that can be cultivated on a large scale or are easy to collect. polysaccharide
In addition, the research on marine natural products is currently mostly focused on small and medium molecules with different structures. Not enough attention has been paid to the polysaccharides rich in most marine organisms, mainly focusing on chitin, algae polysaccharides, etc. [see Noda H, Amano H , Arashima K et al. Nippon Suisan Gakkaishi, 1989, 55: 1265; Zhang EX, Yu LJ, XiaoX. Chinese J Mar Drugs, 1994, 13: 1; Xue CH, Yu GL, Hirata T et al. Biosci Biotechnol Biochem, 1998, 62:206; Liu Xiaomei, Zhang Hongquan. Effects of spirulina polysaccharides on hematopoietic cell proliferation, apoptosis and Bcl-2 expression after chemotherapy in tumor-bearing mice, Acta Pharmaceutica Sinica, 2002, 37:616.], and on the production of marine microorganisms There are very few research reports on new active polysaccharides

Method used

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  • Polysaccharide, preparation and use thereof
  • Polysaccharide, preparation and use thereof
  • Polysaccharide, preparation and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1. Extraction, separation and purification, identification of polysaccharide YCP

[0025] Weigh 1000g of wet bacteria, add water, put it in a tissue masher and mash it, make up water to 2000mL, and extract in a water bath at 60°C for 20 hours. Stir intermittently, filter the residue with gauze, and centrifuge at 2000r.p.m. to remove the precipitate. Concentrate the extract to 500mL, add 1 / 4 volume of chloroform and 1 / 15 volume of n-butanol to the concentrated solution, vibrate vigorously, let stand to separate layers, remove the lower organic solvent layer and the middle layer, and keep the water layer. Repeat the extraction 2 times. The resulting solution was dialyzed against tap water for 24 hours. Concentrate the dialysate to 500 mL, gradually add an equal volume of ethanol, and place at 4°C for 2 hours. After centrifugation, the precipitate was washed successively with absolute ethanol, acetone, and ether, and the crude polysaccharide YCP was dried in vac...

Embodiment 2

[0029] Example 2. Extraction, separation and purification, identification of polysaccharide YCP

[0030] Weigh 1000g of wet fungus, add water, put it in a tissue masher and mash it, make up water to 3000mL, and extract in a water bath at 80°C for 12 hours. Stir intermittently, filter the residue with gauze, and centrifuge at 3000r.p.m. to remove the precipitate. Concentrate the extract to 1000mL, add 1 / 5 volume of chloroform and 1 / 25 volume of n-butanol to the concentrated solution, vibrate vigorously, let stand to separate layers, remove the lower organic solvent layer and the middle layer, and keep the water layer. Extraction was repeated 4 times. The resulting solution was dialyzed against tap water for 36 hours. The dialysate was concentrated to 1000 mL, and 3 times the volume of ethanol was gradually added, and left at 4°C for 6 hours. After centrifugation, the precipitate was washed successively with absolute ethanol, acetone, and ether, and the crude polysaccharide w...

Embodiment 3

[0032] Example 3. Extraction, separation and purification, identification of polysaccharide YCP

[0033] Weigh 1000g of wet bacteria, add water, put it in a tissue masher and mash it, make up water to 4000mL, and extract in a water bath at 90°C for 8 hours. Stir intermittently, filter the residue with gauze, and centrifuge at 4000r.p.m. to remove the precipitate. Concentrate the extract to 2000mL, add 1 / 7 volume of chloroform and 1 / 30 volume of n-butanol to the concentrated solution, vibrate vigorously, let stand to separate layers, remove the lower organic solvent layer and the middle layer, and keep the water layer. Extraction was repeated 5 times. The resulting solution was dialyzed against tap water for 48 hours. The dialysate was concentrated to 2000 mL, and 4 times the volume of ethanol was gradually added, and left at 4°C for 10 hours. After centrifugation, the precipitate was washed successively with absolute ethanol, acetone, and ether, and the crude polysaccharide w...

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Abstract

A polysaccharide consisting glucose and aldonic acid but contains no protein and nucleic acid, whose molecular weight is 1.6X10#+[6]Da~2.6X10#+[6]Da, its terminal group carbons are of alpha-formation, the optical rotation [alpha]=+131.7 deg.-+159.4 deg., the basic matrix of the polysaccharide consists of glucose linked by 1-4 glycosidic bonds, each 17th glucose residue includes a side chain, which is a glucose molecule or an aldonic acid molecule, the proportion of glucose and aldonic acid is 2:1, they are all connected with the main chain glucose through 1-6 glycosidic bonds, the amount of glucose and aldonic acid in each mol of polysaccharide is 53:1. The polysaccharide YCP can be applied into the preparation of medicament for treating tumor. The invention also discloses the process of preparation.

Description

1. Technical field [0001] The invention relates to a polysaccharide, in particular to a polysaccharide YCP of the marine fungus Anthropophyllum polysaccharide and its preparation method and application. 2. Background technology [0002] Fungal polysaccharides are a type of active polysaccharides isolated from fungal fruiting bodies, mycelia, and fermentation broths, which can control cell division and differentiation, and regulate cell growth and aging. The study of fungal polysaccharides as a drug began in the 1950s and attracted people's interest as an immune booster after the 1960s. Fungal polysaccharides are currently recognized as immune enhancers with high effects. Many fungal polysaccharide preparations have been widely used clinically, and have achieved encouraging clinical effects in the treatment of autoimmune diseases, immunocompromised diseases, and tumors. However, the research on polysaccharides of terrestrial organisms (animals, plants, microorganisms, etc.) ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61P35/00C08B37/00
CPCC08B37/006C08B37/00A61P35/00
Inventor 高向东谭仁祥杨晓兵孙诚
Owner CHINA PHARM UNIV
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