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Single cell sensor for ectocytic action potential detection and its preparing method

A potential sensor and action potential technology, which is applied in the field of single-cell sensors and its preparation, can solve the problems of measuring and positioning ion channels, it is difficult to determine the mechanism of drug action, and it is impossible to determine the target of drug action on cells, so as to improve accuracy and Stability, cell attachment and growth are good, and the effect of improving the light source design

Inactive Publication Date: 2004-12-15
ZHEJIANG UNIV
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Problems solved by technology

However, these cell sensors detect the metabolites of the cell population, so it is difficult to determine the mechanism of action of the drug on a single cell. It is an unavoidable difficulty to realize the quantification of drug screening

Method used

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  • Single cell sensor for ectocytic action potential detection and its preparing method
  • Single cell sensor for ectocytic action potential detection and its preparing method
  • Single cell sensor for ectocytic action potential detection and its preparing method

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Embodiment Construction

[0034] 1. The detection structure of the sensor

[0035] Such as figure 1 , figure 2 As shown, the present invention fixes the copper-clad plate 2 on the aluminum plate 1, and the copper-clad plate 2 is adhered with SiO 2 Layer 3.2 is polished thin Si sheet 3.1, and the micro-measurement cavity 7 made of polydimethylsiloxane is fixed on SiO 2 On the layer 3.2, the polydimethylsiloxane micro-measurement chamber 7 is covered with a layer of biocompatible glue 8, which is bonded to the optical glass 4 of the gold-plated reference electrode 5 on the lower surface, and the micro-measurement chamber 7 is coated with more than one layer. Polyornithine and laminin layer 9, the two openings on the glass are respectively connected to the liquid-in latex tube 12 and the liquid-out latex tube 13 of the medicine and the culture solution, and the working electrode 10 is drawn out from the copper-clad plate, and the counter electrode 11 is connected to the gold tube. The membrane referen...

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Abstract

The single cell sensor for ectocytic action potential measurement is developed on the basis of multiple light source cell micro physiological gauge, and has light addressing potential sensor without limitation in cell culture and light source moving addressing capable of tracking and positioning randomly cultured single cell for measurement. The present invention has also improved light source system with raised precision and stability for locating the measurement in single cell or even in single ion passage, so as to measure precisely the action and action target point of medicine on single cell. The single cell sensor can realize the fast real-time monitoring of outer action potential of nerve cell and the qualitative analysis of cell excitation and inhibition of different medicine in different concentration, and is used mainly in medicine screening, analysis and estimation.

Description

Technical field [0001] The invention relates to a single-cell sensor for measuring extracellular action potential and a preparation method thereof. Background technique [0002] In 1992, American Molecular Devices Corporation proposed for the first time to combine living cells with light-addressable potential sensors with EIS (electrolyte / insulating layer / silicon) structure to form a sensitive test instrument to detect changes in the pH of the extracellular microenvironment. On this basis, Some researchers have improved the sensor structure and data processing methods, so that this type of sensor can detect changes in several ions at the same time. However, these cell sensors detect the metabolites of the cell population, so it is difficult to determine the mechanism of action of the drug on a single cell. Realizing the quantification of drug screening is an unavoidable difficulty. Contents of the invention [0003] The object of the present invention is to provide a sin...

Claims

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Application Information

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IPC IPC(8): G01N27/403
Inventor 王平许改霞秦利锋徐莹
Owner ZHEJIANG UNIV
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