Manufacturing method of bracket material for applying in myocardial tissue engineering

A technology of tissue engineering and scaffold materials, applied in tissue culture, bone/connective tissue cells, biochemical equipment and methods, etc., can solve problems such as unsatisfactory quality, and achieve beneficial effects on development and function

Inactive Publication Date: 2005-01-19
INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using these two types of scaffold materials, people have constructed tissue...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0006] Example 1 Preparation of a scaffold that simulates myocardial extracellular matrix

[0007] Collagen comes from pig skin and is mainly composed of types I and III. Commercially available pigskin, remove fat, sundries, etc., shred and dissolve in 0.1% acetic acid. Centrifuge at 2000 rpm to remove undissolved impurities. The collagen in the supernatant solution was precipitated with 4M NaCl. The precipitated collagen was put into a dialysis bag and dialyzed to remove NaCl. The collagen after removing NaCl was dissolved in 0.1% acetic acid again to obtain the desired collagen solution. Collagen and elastin (Sigma Company) were mixed 1:1 to obtain mixture 1, and 20% horse serum (Beijing Yuanheng Sanma Biotechnology Research Institute) and 4% chicken serum were added to 2×DMEM (Gibco BRL or Sigma Company). Embryo extract (self-made) to obtain mixture 2, mixture 1 and mixture 2 were mixed at a ratio of 1:1 to obtain mixture 3, mixture 3 was neutralized with 0.1M NaOH to o...

Embodiment 2

[0008] Example 2 Construction of tissue-engineered myocardium using a scaffold that simulates the extracellular matrix of the myocardium

[0009] Build support material by embodiment 1. Mix freshly isolated neonatal rat (day 1-2) cardiomyocytes with the scaffold material to a final concentration of 2×10 6 / ml or so. The mixed solution was added to the ring tank, and the culture solution (DMEM containing 10% horse serum) was added after 60 minutes. The mixture of cardiomyocytes and collagen can form an annular cardiomyocyte-collagen strip in the annular groove. After the formed ring-shaped cardiomyocyte-collagen strip was cultured in the ring groove for 7 days, it was taken out and stretched with force, and after 7 days, the samples were taken for morphological and histological detection.

[0010] Results: The constructed cardiomyocyte-collagen strips could beat consistently. Histological studies indicated that the cardiomyocytes in the strips formed a tissue-like tissue re...

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Abstract

The invention belongs to the tissue engineering field, more specifically relates to a manufacturing method of stent material for cardiac muscle tissue enginerering. The stent simulates extracellular matrix component. Its original form is a liquid hydrogel. It mainly comprises liquid collagen(I type and III type), elastin, laminin, chicken embryo extractive, 2xDMEM and cow embryo serum. Blending these constituents in proper ratio to obtain natural cardiac muscle analog stent materials and blending them with cardiac cell can generate cardiac cell-bracket materials in various shape, by which a tissue engineered cardiac muscle tissue capable of beating synchronistically can be generated.

Description

technical field [0001] The invention belongs to the field of tissue engineering, and in particular relates to a method for manufacturing a bracket material applied to myocardial tissue engineering. Background technique [0002] Scaffold materials are crucial for constructing tissue-engineered myocardial tissue. The characteristics of cardiomyocytes and myocardial tissue require scaffold materials not only to have good biocompatibility and degradability, but also to be suitable for the growth and development of cardiomyocytes and their physiological functions. high. At present, there are mainly two kinds of scaffold materials for constructing tissue-engineered myocardial tissue, one is various synthetic polymers, such as polylactic acid, and the other is various natural biomacromolecules, such as various collagens. Using these two types of scaffold materials, people have constructed tissue-engineered beating myocardial tissue in vitro, but the quality is still not satisfact...

Claims

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Application Information

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IPC IPC(8): A61L27/00A61L27/60A61L31/04C12N5/077
Inventor 王常勇赵云山郭希民段翠密
Owner INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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