Breast cancer Her-2 immunohisto chemical diagnostic kit

A technology of immunohistochemistry and diagnostic kits, which is applied in the direction of material analysis by observing the influence of chemical indicators, analysis by making materials undergo chemical reactions, scientific instruments, etc., and can solve the problems of troublesome detection and expensive reagents.

Inactive Publication Date: 2005-02-16
LIAONING MEDI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some developing countries have established some effective Her-2 / neu gene and protein detection and evaluation standards to guide and predict the effect of treatment, but the reagents are expensive and the detection is troublesome

Method used

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  • Breast cancer Her-2 immunohisto chemical diagnostic kit
  • Breast cancer Her-2 immunohisto chemical diagnostic kit

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1: The specific operation steps of the present invention for tissue section immunohistochemical staining diagnosis are as follows:

[0036] 1. Synthesis of PAMAM Dendrimer.

[0037] PAMAM Dendrimer is from generation 0 to generation 10, and its molecular diameter is between 10 Å and 130 Å, belonging to nanoscale molecules. Its synthesis adopts the branching method, that is, the molecules grow outward from the core. According to the law of tree branches, the pre-fabricated monomers are combined in a radial order, the branches of the branches are connected to the branches, and the combination is repeated in sequence. With ethylenediamine (EDA) as the initiator nucleus, the first step reaction (a) and methyl acrylate (MA) generate a tetrabasic ester [called 0.5 generation (G)] through Michael addition reaction; the second step reaction (b) The ammonolysis reaction of tetrabasic ester and excess ethylenediamine produces a tetrabasic amide compound (called the f...

Embodiment 2

[0070] This antibody is also suitable for examination of tumor puncture exfoliated cells. Cancer tissue tumor punctured cell smear staining or cell culture on glass slides; punctured cells were evenly spread on glass slides and immediately fixed in 10% neutral formalin solution for 10 minutes after drying. Cell culture on glass slides can be placed directly in fixative for 10 minutes as cancer cells adhere to the slide. After distilled water is cleaned, directly enter the 8th step among the embodiment one, use 0.3% hydrogen peroxide methanol solution (H 2 o 2 ; 1ml: Methanol; 99ml) to block endogenous enzymes, react for 30 minutes at room temperature and wash with PBS 3 times. The following other steps are the same as in Embodiment 1.

Embodiment 3

[0072] This kit can also be used to detect the expression of Her-2 in other various cancer tissues. Same method

[0073] Embodiment one.

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Abstract

The present invention relates to a Her-2 immuno-histochemical diagnostic kit of mammary cancer. In its content the non-specific protein blocking agent, raat antihuman initial antibody, sheep antirat monoclonal connection antibody, enzyme-labeled PAMAM reagent and color-developing reagent prepared by using chromographorous oxidation reaction agent, developing colour agent and colour-developing buffer solution. Said invention utilizs the biological characteristics of PAMAM dendritic multimer to label antibody to implement Her-2 immuno-histochemical diagnosis of mammary cancer. Said invented kit can provide accurate, stable and effective diagnosis for mammary cancer and early tumor.

Description

technical field [0001] The invention relates to a clinical tumor diagnosis kit, in particular to a kit for immunohistochemical diagnosis of Her-2 in breast cancer by utilizing the biological characteristic marker antibody of PAMAM dendritic polymer. It can also be used to detect the expression of Her-2 in the diagnosis of various other cancer tissues. Background technique [0002] In recent years, the number of breast cancer patients is rising sharply, and the incidence rate ranks first among female tumors. About 50,000 to 90,000 women in my country develop breast cancer every year, and 30,000 to 40,000 women die of breast cancer. In large coastal cities such as Shanghai, the incidence of breast cancer ranks first among female tumors. About 120,000 to 150,000 breast cancer patients need observation and treatment every year. Among all breast cancer patients, 25-30% have overexpression of Her-2 / neu (epidermal growth factor receptor) protein, and this type of tumor is more m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N33/532G01N33/535G01N33/536
Inventor 李文欣
Owner LIAONING MEDI BIOTECH CO LTD
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