Porphyra conchocelis pure line making method

A filamentous and thallus technology, which is applied in the field of seed production of the filamentous pure line of Porphyra chinensis in North China, can solve the problems of inability to obtain filiform germplasm, inability to determine the genetically pure line of the filamentous body, etc.

Inactive Publication Date: 2005-03-16
OCEAN UNIV OF CHINA
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Problems solved by technology

The common feature of the above two methods is that it is impossible to determine whether the obtained filame

Method used

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Embodiment Construction

[0009] The inventor brought back the Porphyra frondosa individuals from the intertidal zone of Taipingjiao in Qingdao in the spring of 2001, and picked out hermaphroditic, all-female and all-male individuals through preliminary sorting; Individuals, use absorbent cotton balls in filtered seawater for preliminary cleaning to remove the main attached organisms on the surface of the algae. Generally, it needs to be washed 2 to 3 times; spread the picked algae on paper or a petri dish and drain Excess water, because North China half-leaf laver is not resistant to drying, special attention should be paid to the operation time should not be too long to avoid the death of algae cells due to excessive water loss; or when the algae is about to dry, use a dropper to take a small amount of clean water. Drop seawater on the algal body; then cut off small pieces of vegetative tissue (generally 1-2mm square) with a clean blade. For hermaphroditic individuals, the selected position is general...

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Abstract

Disclosed is a method for production of hybrid seeds of laver filament pure lines characterized in that the method comprises the steps of screening individuals with dissimilar sex character from feral population, scrubbing with absorbent cotton balls, filtering excess seawater, cutting down 1-2mm squared algae slices on the base portion of the hermaphroditic algae, confirming that all the cells are nurse cells, supersonic cleaning, culturing under the condition of 10-14 hours light lamination, and separating single algae for expansion.

Description

technical field [0001] The invention relates to a seed production method of a pure line of Porphyra chinensis filaments. Background technique [0002] Porphyra is an important economical seaweed, but only a few species are cultivated, and they are mainly concentrated in Southeast Asian countries such as China, Japan, and Korea. Porphyra half-leaf in North China is a unique type of laver in the intertidal zone of the Yellow Sea and Bohai Sea in northern my country. It has not yet become a cultivated species, but because its algae are bright purple or reddish brown, compared with common species in my country, it may contain more Phycobiliprotein; and its algae have good luster and resistance to adhesion. The growing season is late winter and early spring, and artificial seedling cultivation can be carried out in areas with low temperature, so it has high potential economic value. [0003] The thallus of Porphyra is the gametophyte stage, while the easy-to-preserve germplasm is...

Claims

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Application Information

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IPC IPC(8): A01G33/02A01H4/00
Inventor 汤晓荣
Owner OCEAN UNIV OF CHINA
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