Porphyra conchocelis pure line making method

Abstract

Disclosed is a method for production of hybrid seeds of laver filament pure lines characterized in that the method comprises the steps of screening individuals with dissimilar sex character from feral population, scrubbing with absorbent cotton balls, filtering excess seawater, cutting down 1-2mm squared algae slices on the base portion of the hermaphroditic algae, confirming that all the cells are nurse cells, supersonic cleaning, culturing under the condition of 10-14 hours light lamination, and separating single algae for expansion.

Description

technical field [0001] The invention relates to a seed production method of a pure line of Porphyra chinensis filaments. Background technique [0002] Porphyra is an important economical seaweed, but only a few species are cultivated, and they are mainly concentrated in Southeast Asian countries such as China, Japan, and Korea. Porphyra half-leaf in North China is a unique type of laver in the intertidal zone of the Yellow Sea and Bohai Sea in northern my country. It has not yet become a cultivated species, but because its algae are bright purple or reddish brown, compared with common species in my country, it may contain more Phycobiliprotein; and its algae have good luster and resistance to adhesion. The growing season is late winter and early spring, and artificial seedling cultivation can be carried out in areas with low temperature, so it has high potential economic value. [0003] The thallus of Porphyra is the gametophyte stage, while the easy-to-preserve germplasm is...

AI Technical Summary

Problems solved by technology

The common feature of the above two methods is that it is impossible to determine whether the obtained filaments are genetically pure lines, and it is impossible to obtain filamentous germplasm from all-male individuals