Biological method for preventing and treating white ant
A technology of termites and domestic termites, applied in botany equipment and methods, biocides, animal repellents, etc., can solve the problems that the work of termite control has not been reported.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
example 1
[0020] Example 1: The cultivation and formulation of Xenorhabdus nematophilus and Photorhabdus luminescens bacteria, and Metarhizium anisopliae, used in Examples 2-7.
[0021] Cultivation and Formulation of Bacteria of the Genus Xenorhabdus and Photorhabdus:
[0022]Add 100mL liquid medium (1% peptone, 0.5% NaCl) to a 500mL Erlenmeyer flask, and autoclave at 121°C for 30 minutes; then insert 1mL of X.nematophilus or P.luminescens primary symbiotic strains, and place at 25°C , Shaker culture under 150r / min for 48 hours, the obtained bacterial liquid (about 10 per milliliter 7 ~10 8 Bacteria) can be used as water agent for termite control. Such bacteria can also be cultured in large quantities in fermenters or reactors. When using bacterial liquid, personnel in this professional field can dilute the bacterial liquid to 10% per milliliter according to the occurrence and quantity of termites. 5 ~10 8 The bacterial range is then applied to control termites. However, the optim...
example 2
[0027] Example 2: Mixed use of pathogenic bacteria liquid and pathogenic fungus spore liquid to prevent and control house termites.
[0028] After X.nematophilus bacterium or P.luminescens bacterium are cultured in liquid medium (1% peptone, 0.5% NaCl), obtain bacterial liquid (about every milliliter 5 * 10 8 bacteria). After culturing Metarhizium anisopliae in potato dextrose medium (PDA) in a culture room at 25°C for 10 days, the plate was covered with hyphae and produced spores. The long spores in the plate are washed with sterile water to make a liquid preparation (about 5 × 10 per milliliter). 7 spore). Add a layer of medium-speed filter paper and 1 g of wood chips (also autoclaved) with a water content of 10% to each autoclaved plate (diameter = 9 cm), take 0.5 ml of bacterial bacteria liquid and 0.5 ml of fungal spore liquid and mix well , Then add in the sawdust, then add 20 worker ants that are not infected by bacteria and fungi in the flat plate. Add only bacteri...
example 3
[0031] Example 3: Mixed use of pathogenic bacteria and pathogenic fungus powder to control domestic termites.
[0032] Adding one deck of medium-speed filter paper and 1 gram of water content is 10% sawdust (also through autoclaving) in the flat plate (diameter=9cm) of each autoclaving, according to the powder prepared as described in Example 1, get 0.5 gram of bacterial powder (approximately 10 per gram 8 bacteria) and 0.5 grams of fungal spore powder (containing about 10 per gram 8 spores) were mixed evenly, then added in sawdust, and then added 20 worker ants that were not infected by bacteria and fungi in the flat plate. Only bacteria powder, or only fungal spore powder was added to the plate, or termites not exposed to biological agents were placed in the same petri dish as a control. Each treatment was replicated with 4 plates. All plates were sealed with Parafilm and placed at 25°C. The death of termites was checked every day, and the number of dead termites was rec...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More