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Process for preparing water-soluble chitosan oligosaccharide

A technology for water-soluble chitosan oligosaccharide and chitosan is applied in the field of preparation of water-soluble chitosan oligosaccharide, and can solve the problems of low molecular weight, unsuitable application, unfavorable industrial production and the like

Active Publication Date: 2005-08-17
山东贝科德糖生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Existing methods for preparing water-soluble chitosan oligosaccharides mainly include enzymatic method and acid method. Among them, the enzymatic method has the disadvantages of low molecular weight, long reaction time, and is not conducive to industrial production. The acid method has long reaction time, difficult control of molecular weight, and acid residues. Difficult to remove, unsuitable for food applications

Method used

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  • Process for preparing water-soluble chitosan oligosaccharide
  • Process for preparing water-soluble chitosan oligosaccharide

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specific Embodiment approach 1

[0013] Specific Embodiment 1: In this embodiment, water-soluble oligochitosan is prepared according to the following steps: a. After drying and crushing the clean crab shells, soak them in an acidic solution for 5-8 hours; b. After washing, soak them in an alkaline solution Boil for 1-2 hours, wash until neutral to obtain chitin; c, then dissolve chitin in alkaline solution, heat at 100-110°C for 4-8 hours, wash once every 2 hours, and wash until the last time Neutral to obtain chitosan; d, chitosan is washed, dried and dissolved in acetic acid solution, according to the weight ratio of acetic acid solution: chitosan: hydrogen peroxide=20~30: 1: 2~4 Add hydrogen peroxide in an appropriate amount, hydrolyze for 80-100 minutes at a temperature of 60-80°C; e, then concentrate in a vacuum at a temperature of 30-50°C, filter with suction, and finally neutralize with an alkaline solution, vacuum freeze-dry, and seal , to obtain water-soluble chitosan oligosaccharides. Described aci...

specific Embodiment approach 2

[0014] Specific embodiment two: this embodiment prepares water-soluble chitosan oligosaccharides according to the following steps: a, after drying and pulverizing the clean crab shells, soak them in HCl with a volume concentration of 10% for 6 hours, and wash once every 3 hours; b, After washing, boil in a NaOH solution with a weight concentration of 10% for 1.5h, wash to neutrality, and obtain chitin; c, dissolve chitin in a sodium hydroxide solution with a weight concentration of 55%, and heat Heating under heating for 6 hours, washing once every 2 hours, and washing to neutrality for the last time to obtain chitosan; d, washing and drying chitosan and dissolving it in an acetic acid solution with a volume concentration of 2%, according to the weight ratio Add hydrogen peroxide in the amount of acetic acid solution: chitosan: hydrogen peroxide=25:1:3, and hydrolyze for 90min at a temperature of 70°C; NaOH solution neutralization, vacuum freeze-drying and sealing to obtain wa...

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Abstract

The preparation process of water soluble chitosan includes the following steps: a) stoving and crushing clean crab shell and soaking in acid solution; b) washing, boiling in alkali solution and washing to neutrality; c) dissolving chitin in alkali and heating to obtain chitosan; d) washing and stoving chitosan, dissolving in acetic acid solution, and hydrolyzing with excessive hydrogen peroxide; and e) vacuum concentration, suction filtering, and neutralizing with alkali solution to obtain water soluble chitosan. The present invention has the advantages of simple technological process, low production cost, safe product without side effect, and excellent physiological activity and health care effect of the product.

Description

Technical field: [0001] The invention relates to a preparation process of water-soluble chitosan oligosaccharide. Background technique: [0002] Chitin is a 1,4-linked 2-acetylamino-2-deoxy-β-D-glucose, which is widely found in the shells of insects, crustaceans and fungal cell walls, and is second only to cellulose in nature. natural polysaccharide, but its application is limited due to its poor solubility. [0003] Chitosan (chitosan) is a derivative of chitin deacetylation, which is composed of most of D-glucosamine and a small amount of N-acetyl-D-glucosamine, connected by β-(1,4) glycosidic bonds The linear polysaccharide of β-glucose has the chemical name (1,4)-2-amino-2-deoxy-β-D-glucose. The presence of hydroxyl, acetamido and amino groups in the structure of chitosan determines that chitosan can undergo chemical reactions of various functional groups. Chitooligosaccharides are a class of oligomers produced by hydrolysis of chitosan. These oligomers have high solu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08
Inventor 王静曹维强赵海田
Owner 山东贝科德糖生物科技有限公司
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