Process for producing nerve cells

A technology of nerve cells and neural stem cells, which is applied in the field of treatment of neurodegenerative diseases or nerve damage, and can solve problems such as being unsuitable for a large number of neural stem cells, difficult for neural stem cells, and slow in proliferation of neural stem cells.
CN1662645AInactive Publication Date: 2005-08-31MITSUBISHI TANABE PHARMA CORP
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
MITSUBISHI TANABE PHARMA CORP
Publication Date
2005-08-31
Estimated Expiration
Not applicable · inactive patent

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Abstract

To supply substantially isolated neural cells in a large amount, and to provide an application means for a neurodegenerative medicine or the like for a neurodegenerative disease, a nervous damage or the like. A method for producing a substantially isolated neural cell, comprising the step of carrying out the suspension culture of embryonic stem cells in the presence of an astrocyte conditioned medium or ingredients substantially equivalent to the conditioned medium; and a neural cell obtained thereby; a cell pharmaceutical composition comprising, as an active ingredient, the isolated neural stem cell; and a method for treating a neurodegenerative disease or nervous damage, comprising the step of introducing the neural cell into a neurodegenerative site or a nervous damage site.
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Description

technical field

[0001] The present invention relates to a method for producing substantially isolated neural cells, substantially isolated neural stem cells, substantially isolated neurons, substantially isolated glial cells, neural cells of cell pharmaceutical compositions, and neurodegenerative diseases or treatments for nerve damage. Background technique

[0002] At present, the production of nerve cells from embryonic stem cells is mainly carried out by the following four methods.

[0003] ① A method of treating suspension cultures of embryonic stem cell aggregates with retinoic acid and inducing their differentiation into various nerve cells [Bain, G. et al., Dev. Biol., 168: 642-657 ( 1995)]

[0004] ② After preparing embryoid bodies [called EBs] and culturing the EBs with serum-free medium to obtain neuroepithelial stem cell protein-positive neural stem cells, in the presence of basic fibroblast growth factor (bFGF), A method for culturing the neural stem cells and...

Claims

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