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Outer space carrying device for gene engineering cell

A genetic engineering and cell technology, applied in the field of space carrying devices, can solve the problem of difficult to provide living conditions for mammalian cells

Inactive Publication Date: 2005-09-21
CHINA JAPAN FRIENDSHIP HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since it is difficult to provide living conditions for mammalian cells by passively carrying returnable satellites, it limits the research and application of mammalian genetically engineered cells carrying returnable satellites for space mutagenesis to increase the yield of genetically engineered drugs

Method used

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  • Outer space carrying device for gene engineering cell
  • Outer space carrying device for gene engineering cell
  • Outer space carrying device for gene engineering cell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] see Figure 1-2 As shown, the container of this device adopts a cryopreservation tube 2 made of polypropylene material, and a synthetic culture solution 4 is housed in it. Add glutamine in a ratio of 2 grams; add hydrocortisone in a ratio of 1 milligram per liter, and be immersed in the microcarrier 3 with holes in the synthetic culture solution, the particle component is cellulose, and the particle diameter is 230 Micron, the pore size is 30 microns, add dihydrofolate reductase-deficient Chinese hamster ovary cells (CHO-dhfr - ), screw in the cap 1 for sealing, and maintain the cells to survive at 4-37°C for 30 days at room temperature, after which they can continue to grow and reproduce, see image 3 shown.

Embodiment 2

[0023] see Figure 1-2 As shown, the device container adopts a cryopreservation tube 2 made of polypropylene material, and a synthetic culture solution 4 is housed in it. Add glutamine in a ratio of 2 grams; add hydrocortisone in a ratio of 1 milligram per liter, and be immersed in the microcarrier 3 with holes in the synthetic culture solution, the particle component is cellulose, and the particle diameter is 230 Micron, the pore size is 30 microns, add dihydrofolate reductase-deficient Chinese hamster ovary cells (CHO-dhfr - ), screwed into the cover 1 to seal it, carried China's 18th returnable satellite, after 17 days of space flight and 8 days of ground stay, the cells were kept alive for 25 days in total, and the cells can continue to grow and reproduce at 4-37°C ,See Figure 4 shown.

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Abstract

This invention relates to a gene engineering cell embarking device, which comprises container(2), top cover(1) of the container, culture liquid(4) in container, and microcarrier(3) with pore immersed in the liquid, characterized in that the microcarrier is non-poisonous high molecular compound selected from cellulose, crust or collagen, with a 20-60micrometre diameter pore on it, and the culture liquid is medium added glutamine and hydrocortisone. The device can sustain cells alive for 30 days under the conditions of non-power, non-heat, non-oxygen, non-carbon dioxide, and no need of renewing medium, and when cells get back to normal temperature from 4-37Deg.C, it can still grow and reproduce at 36.5Deg.C, with 0.5Deg.c more or less.

Description

technical field [0001] The invention relates to a device for carrying genetically engineered cells in space, in particular to a device for long-term survival of genetically engineered cells that are passively carried by returnable satellites. Background technique [0002] The space environment has many characteristics different from those on the earth's surface, such as microgravity, micromagnetic field, space radiation, etc. These characteristics can affect life on earth in many ways. Since 1987, our country has used returnable satellites to carry plant seeds, and through mutation breeding in the space environment, a variety of high-quality, high-yielding crops and high-product lines have been cultivated on the ground. my country has carried a variety of antibiotic strains on returnable satellites, and the production of antibiotics has increased significantly. Among them, in 1994, the cephalosporin acylase genetically engineered strain in the proliferating phase was carrie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00
Inventor 房青唐劲天向青陈志华徐梅李红艳周东辉
Owner CHINA JAPAN FRIENDSHIP HOSPITAL