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Diwu saponin W3 standard product extracted from Linyin anemone and preparing process

A kind of technology of kinarin and diwu, applied in the field of diwusaponin W3 standard product and preparation process

Inactive Publication Date: 2005-10-19
湖北中医学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no diguanin W 3 Standard product, as a quality standard for controlling related Diwu medicinal materials, Diwu extracts and preparations

Method used

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  • Diwu saponin W3 standard product extracted from Linyin anemone and preparing process

Examples

Experimental program
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Effect test

Embodiment 1

[0017] Extract and isolate diguanin W from Diwu 3 process such as figure 1 As shown, take Diwu coarse powder, heat and reflux extraction with 70-95% ethanol for 3 times, each time for 3 hours, combine the extracts, concentrate to dryness under reduced pressure at 60°C, and obtain dry extract; suspend the dry extract in water , extracted 3 times with water-saturated n-butanol, and the n-butanol layer was concentrated to dryness under reduced pressure to obtain n-butanol dry extract; , 40-80% ethanol, 80-95% ethanol elution; collect 40-80% ethanol eluate, reclaim ethanol and evaporate to dryness in a water bath to obtain diglucoside; carry out silica gel chromatography with chloroform:methanol : Water was eluted with a mixture of 20:10:1 volume ratio, roughly divided into 5 sections, detected by TLC, and collected rich in diguanin W 3 Part of the second paragraph, then silica gel chromatography, eluted with a mixture of chloroform:methanol:water at a volume ratio of 7:4:1, TLC...

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Abstract

The present invention relates to compound extracted from flaccid anemone, 3-O-alpha-L-pyranorhamnose (1->2)-beta-D-xylopyranose-oleanolic acid-28-O-alpha-L-pyranorhamnose(1->4) beta-D-pyranoglucose (1->6) beta-D-glucopyranoside, named flaccid anemone saponin W3, and with molecular expression of C59H96O25 and purity over 98 %. Flaccid anemone saponin W3 is prepared with coarse flaccid anemone rhizome powder, and through ethanol or methanol extraction, concentration, elution in macroposrous resin, twice silicon gel chromatographic separation and twice elution. Flaccid anemone saponin W3 may be sued as W3 standard used in controlling the quality of flaccid anemone medicine material, flaccid anemone extract and flaccid anemone preparation.

Description

technical field [0001] The invention relates to a standard product and an extraction and separation process, in particular to a standard product of diwusaponin W3 extracted from Diwu diwu and a preparation process thereof. Background technique [0002] The dry rhizome of Anemone flaccida Fr. Schmidt, a plant of the genus Anemone in the Ranunculaceae family of Diwu (Dictionary of Traditional Chinese Medicine, Shanghai: Shanghai Science and Technology Press, 2002: 802). Diguanin W 3 It is extracted, separated and purified from Diwu. At present, there is no diguanin W 3 The standard product is used as the quality standard for controlling related Diwu medicinal materials, Diwu extracts and preparations. [0003] Diguanin W 3 The preparation of the standard is to use silica gel chromatography as a rough separation method, which is an advanced method. Compared with the silica gel column separation method, the speed is fast; the preparation and separation of the preparative liq...

Claims

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Application Information

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IPC IPC(8): C07H1/08C07H17/07C07J63/00
Inventor 邴飞虹韩林涛
Owner 湖北中医学院
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