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Hybridized tumour cell strain of anti hpatitis B virus surface antigen and its monoclonal antibody

A technology of hybridoma cell lines and monoclonal antibodies, applied in the direction of anti-animal/human immunoglobulin, microorganisms, biochemical equipment and methods, etc.

Inactive Publication Date: 2005-11-09
TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, many research institutes have prepared relevant monoclonal antibodies that can be used in clinical testing, but they are mainly used to detect wild strains, and monoclonal antibodies that can better identify mutant strains have not been reported so far

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0029] 1. Establishment of anti-HBsAg monoclonal antibody cell lines

[0030] 1. Preparation of immunogen

[0031] The immunogen is HBsAg isolated from the serum of HBV-infected patients.

[0032] 2. Immunized animals:

[0033] Take 20ug of HBsAg and dilute it in 100ul of normal saline, add an equal amount of aluminum hydroxide adjuvant, mix well, and inject it subcutaneously at multiple points on the neck and back of BALB / c mice (6 weeks old). After an interval of 2 and 4 weeks, the same amount of antigen plus incomplete adjuvant was used for booster immunization twice. After the immunization, a little mouse blood was collected to detect the HBsAb titer. Two days before fusion, the same dose of antigen was injected intraperitoneally for booster immunization.

[0034] 3. Immunological titer detection

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Abstract

A hybridoma cell strain (CCTCC-C200505) is disclosed, which can secrete the monoclonal antibody of HBV surface antigen. An EL1SA method created by enveloping the monoclonal antibody can detect wild-type HBsAg and 13 variant HBsAgs.

Description

technical field [0001] The invention relates to a biological preparation, in particular to a hybridoma cell line secreting a monoclonal antibody against the surface antigen of hepatitis B virus (Hepatitis B surface antigen, HBsAg) and the secreted monoclonal antibody. technical background: [0002] HBsAg is an important marker for the diagnosis of hepatitis B virus infection. At present, most domestic and foreign HBsAg detection kits are ELISA detection methods established by using anti-HBsAg monoclonal antibody or polyclonal antibody. The antibodies used in various kits are different, and the ability to detect mutant hepatitis B virus is different. The current research results show that domestic HBsAg detection kits have poor ability to detect mutant strains, mainly because there is no monoclonal antibody that can recognize the mutant strains well. Therefore, it is necessary to develop monoclonal antibodies that can detect wild and variant HBsAg, and establish a detection...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/18
Inventor 杨东亮李方和田拥军张振华龚劲松
Owner TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH
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