Lyophilized powder injection for treating ischemic cardio-cerebrovascular diseases and its preparing method
A technology for cardiovascular and cerebrovascular diseases and freeze-dried powder injection, which is applied in the field of preparation of traditional Chinese medicines, can solve problems such as preparations for cardiovascular and cerebrovascular diseases that have not been seen on the market, and achieves easy control of product quality, vasodilation, efficacy and safety. high sex effect
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preparation example Construction
[0126] Preparation of Ginkgo biloba extract: Take 1 kg of dry green Ginkgo biloba coarse powder, extract 3 times with 8 times the amount of 60% ethanol under reflux, each time for 1.5 hours, combine the extracts, filter, reclaim the ethanol from the filtrate, add methionine to stir and dissolve, and use Citric acid was used to adjust the pH value to 4-5, concentrated to liquid extract (1:1.5), filtered, the filtrate was concentrated to (1:5.5), extracted 3 times with n-hexane, and the extracted aqueous phase was extracted with ethyl acetate for 4 times, and then extracted 6 times with water-saturated sec-butanol-ethyl acetate, combined all extracts, recovered ethyl acetate and sec-butanol under reduced pressure at about 50°C and 80°C, respectively, evaporated to remove residual ester and residual alcohol, Polyamide chromatography, eluting with 1000 ml of 60% ethanol, combining the eluents, recovering ethanol, concentrating to near dryness, and vacuum drying at 70°C to obtain th...
Embodiment 2
[0128] Example 2 of product preparation: Take about 1600ml of water for injection and put it in a concentrated preparation container, add about 165g of mannitol, heat and stir to dissolve, adjust the pH to 4-5 with 10% citric acid solution, add 0.2% activated carbon for 15 minutes, filter To clarify, add 20g of Ginkgo biloba extract to the filtrate, stir to dissolve, filter to clarify with a composite micromembrane filter, add water for injection to 1800ml, fine filter with a stainless steel micromembrane terminal filter to clarify, aseptically subpackage, pre-freeze, freeze-dry, seal, Promptly get 1000 bottles of freeze-dried powder for injection.
Embodiment 3
[0129] Example 3 of product preparation: Take about 1600ml of water for injection and put it in a concentrated preparation container, add about 165g of mannitol, heat and stir to dissolve, adjust the pH to 4-5 with 10% citric acid solution, add 0.2% activated carbon for 15 minutes, filter To clarify, add 55g of Ginkgo biloba extract to the filtrate, stir to dissolve, filter to clarify with a composite micromembrane filter, add water for injection to 1800ml, fine filter with a stainless steel micromembrane terminal filter to clarify, aseptically subpackage, pre-freeze, freeze-dry, seal, Promptly get 1000 bottles of freeze-dried powder for injection.
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