Particle analyzing chip with microflow control of single-cell algae

A microfluidic chip, particle size analysis technology, applied in the direction of individual particle analysis, particle and sedimentation analysis, analysis materials, etc., can solve the problems of high price, large volume, not suitable for on-site analysis, etc., to improve detection sensitivity and ensure stability. sexual effect

Inactive Publication Date: 2005-12-28
OCEAN UNIV OF CHINA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the current Coulter counter is expensive and bulky, and is not suitable for on-site analysis. The emergence of microfluidic chips provides the possibility for the on-site application of Coulter technology

Method used

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  • Particle analyzing chip with microflow control of single-cell algae
  • Particle analyzing chip with microflow control of single-cell algae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Structure of microfluidic chip

[0024] Such as figure 1 As shown, the structure of the microfluidic chip is divided into two parts: the liquid flow system and the detection system. The liquid flow system adopts a ψ-shaped structure, the middle channel is the sample channel 7, and the side branch channels are the sheath flow channels 8 and 9. The function of the sheath flow channel is to arrange the algae cells in the sample in a single row. 8 and 9 have the same size, the sheath flow channel is a 1 / 4 arc shape, and the tangential direction of the sheath flow channel 8 and 9 at the intersection point 10 is consistent with the direction of the sample channel 7, so that the sheath flow and the sample flow merge in the same direction, avoiding The eddy current disturbance generated by the convergence of the liquid flow ensures the stability of the liquid flow system; a double "T"-shaped detection channel 11 perpendicular to the sample channel ...

Embodiment 2

[0025] Example 2: Fabrication of microfluidic chip

[0026] 1. Fabrication of the glass substrate: Dimension design of the microchannel on the mask film: the fluid channel, 30 μm; the detection channel, 30 μm, 300 μm, with a width of 5 mm and a narrow length of 0.2 mm; the length of the common channel is 0.35 mm. Place the mask film on a 63mm x 63mm x 1.5mm uniform chromium plate, expose it to ultraviolet light for 180 seconds (wavelength 365nm), develop it in a developer for 100 seconds, and then dry it at 100°C for half an hour. The chromium film was etched with a chromium film etching solution (cerium sulfate: perchloric acid: water=50 g: 15 ml: 300 ml) at room temperature, then rinsed with high-purity water, and dried. The dimensions of the channels on the chromium plate measured by digital microscope photography are: sample channel, 40 μm; detection channel, 40 μm, 310 μm. With 0.5M HF / 0.5M NH 4 F etchant etches the bare borosilicate glass at a rate of...

Embodiment 3

[0029] Example 3: Microfluidic chip analysis and detection system

[0030] Such as figure 2 As shown, the inlet 1 of the sample channel 7 is connected to the sample pump 13, the sheath flow channels 8 and 9 on both sides are connected to the sheath flows 15, 16 at their inlets 2 and 3, electrodes are arranged at the two ends 4, 5 of the detection channel 11 and It is connected to a constant current meter to form the whole microfluidic chip analysis and detection system. The sample and the sheath fluid are transported by the pumps 12 and 13, the sheath fluid flows at the same flow rate, and the sheath fluid flows forward in the form of laminar flow after converging with the sample. By adjusting the sheath flow and sample flow rate, the cells in the sample are Arranged in a single row and move forward through the detection area (the common area of ​​the detection channel and the sample channel), the cells in the detection area replace the equal volume of electrol...

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Abstract

A mini-flow control chip of mono-cell alga size analysis is prepared by having substrate and cover-plate in symmetric structure, setting psi form liquid flow system formed by intermediate sample channel and sheath channel as well as setting double T form detection channel on both of substrate and cover-plate, setting electrode at two sides of detection channel with constant current for obtaining potential difference to change pulse signal when cell passes through detection channel, then calculating size and number of cell as per pulse frequency and intensity .

Description

technical field [0001] The invention relates to a particle size analysis instrument designed and manufactured by the Coulter principle, more specifically a microfluidic chip used for particle size analysis of marine unicellular algae. Background technique [0002] The introduction of the concept of Micro-total analysis system (μ-TAS) has had a great impact in the field of analytical science, leading the development of chemical analysis equipment towards the trend of miniaturization, integration and portability. It utilizes micromachining technology to fabricate functional units such as microvalves, micropipes, microreactors, microflow sensors, and microdetectors on the chip to form a microchemical system. The microfluidic chip system has the characteristics of high efficiency, low consumption, miniaturization and integration, and is suitable for various on-site analysis and real-time determination. In 1995, Mathies and Woolley first used micro-electrophoresis chips to condu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/12
CPCG01N15/12G01N15/1056G01N2015/1062G01N2015/1087
Inventor 王修林苏荣国韩秀荣
Owner OCEAN UNIV OF CHINA
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