Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Fermentation technology of insect pathogenic nematode symbiotic bacteria and application of its fermented liquid

A technology of entomopathogenic nematodes and fermentation technology, which is applied in the application, bacteria, biocide and other directions to achieve the effect of low production cost and good application prospect.

Inactive Publication Date: 2006-01-25
HARMLESS PESTICIDE RES & SERVICE CENT XIBEI AGRI FORESTRY SCIEN
View PDF0 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are no reports or patents on the related technologies and methods of the cultivation and fermentation of symbiotic bacteria and the production of secondary metabolites

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Streak the pathogenic bacteria Xenorhabdus nematophilus YL001 stored in the seed tube on the NA medium plate, culture at 28°C for 24 hours, pick a single colony, then streak on the NBTA medium plate, and cultivate at 28°C for 48h;

[0030] 2. Use an inoculation loop to pick up the blue colony on the NBTA medium plate, inoculate it in a 250ml Erlenmeyer flask filled with 50ml NB medium, and culture it with shaking at 28°C and 180r / min for 16-24h to become a first-grade seed solution;

[0031] 3. Put the first-grade seed solution into a 250ml Erlenmeyer flask containing seed medium at an inoculation amount of 9.1%, the liquid volume is 25ml, pH7.25, shake and cultivate at 26°C and 220rpm for 16-24h, and become a second-grade seed liquid;

[0032] 4. Add 4.5L fermentation medium, 0.01% defoamer, pH 7.2, 0.1-0.15MPa off-site steam sterilization for 30min to 28°C in 7L stirred fermenter, add 9.1% secondary seed liquid, Under the conditions of 26° C., ventilation rate 2.5...

Embodiment 2

[0040] 1. Streak the pathogenic bacteria Xenorhabdus nematophilus YL001 stored in the seed tube on the NA medium plate, culture at 28°C for 24 hours, pick a single colony, then streak on the NBTA medium plate, and cultivate at 28°C for 48h;

[0041] 2. Use an inoculation loop to pick up the blue colony on the NBTA medium plate, inoculate it in a 250ml Erlenmeyer flask filled with 50ml NB medium, and culture it with shaking at 28°C and 180r / min for 16-24h to become a first-grade seed solution;

[0042] 3. Put the first-grade seed solution into a 250ml Erlenmeyer flask containing seed medium at an inoculation amount of 9.1%, the liquid volume is 25ml, pH7.25, shake and cultivate at 26°C and 220rpm for 16-24h, and become a second-grade seed liquid;

[0043] 4. Add 45L fermentation medium, 0.01% defoamer, pH 7.2, 0.1-0.15MPa steam sterilization to 70L stirring fermenter for 30 minutes, cool to 26°C, add 9.1% of secondary seed liquid, at 26°C, Under the conditions of ventilation r...

Embodiment 3

[0052] The application of Xenorhabdus nematophilus YL001 fermented liquid of Xenorhabdus nematophilus YL001 carries out on the basis of embodiment 1,2:

[0053] 1. The fermentation broth obtained in the process is further centrifuged at 14000r / min, 4°C for 10min, and the supernatant is collected;

[0054] 2. Dilute the supernatant and fermented product 10 times with the melted PDA medium, put 10ml of the medium into a sterilized petri dish with a diameter of 9.0cm to make a plate, and use the PDA with sterile medium as a control . From the edge of the phytopathogenic fungal colonies cultivated for 3 to 6 days, use a puncher to cut into 7.5mm-diameter bacterial blocks, place them in the center of the plate, and cultivate them in the dark at 20°C for 3 days to measure the diameter of each pathogenic fungal colony;

[0055] 2. The supernatant of Xenorhabdus nematophilus YL001, at a dose of 300-500ml / L, has a strong effect on the mycelium growth of Alternaria tabacum, tomato earl...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an application of fermenting technology and fermenting material of entomiasis initial line insect symbiosis fungus. It uses Xenorhabdus nematophilus YL001 as raw material and builds a fermenting technology that could improve the antibacterial activity and high biological yield. The fermenting product of Xenorhabdus nematophilus YL001 could be made into entomiasis initial line insect symbiosis fungus antiseptic for controlling cayenne pestilence mildew, and the effect is good.

Description

technical field [0001] The invention relates to a bacterial fermentation process and the application of the fermented product in the prevention and control of crop diseases, in particular to the fermentation process of the pathogenic bacteria nematophila and the application of its fermented liquid in the preparation of fungicides for the prevention and control of crop diseases. Background technique [0002] Entomopathogenic nematode symbionts are a type of bacteria parasitic in the intestinal tract of entomopathogenic nematodes, belonging to the family Enterobacteriaceae, facultatively anaerobic, chemoheterotrophic, and Gram stain negative. Including Xenorhabdus and Photorhabdus, they live in symbiosis with Steinernema and Heterorhabditis nematodes respectively. In nature, such bacteria exist in the intestines of nematodes at the third instar infection stage. As the nematodes infect insects, the symbiotic bacteria are carried into the insect and r...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02
Inventor 张兴王永宏李骞马志卿李广泽吴芳丽郝双红何军
Owner HARMLESS PESTICIDE RES & SERVICE CENT XIBEI AGRI FORESTRY SCIEN
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com