Fluorescence detection optical device with microflow control chip

Abstract

The invention is a micro-flow control chip fluorescent detection optical device, comprising light source, filter plate, semitransparent semi-reflector, object lens group and photoelectric multiplier tube, and the structure of the detection optical device includes detecting light path, information collection interface and control interface. The invention is wide-application range, and flexible to adjust, the object lens group and the micro-channel of the micro-flow control chip automatically focus and the data processing is automatically completed by computer, quick and high-accuracy.

Description

technical field [0001] The invention relates to fluorescence detection of a microfluidic chip, in particular to an optical device for fluorescence detection of a microfluidic chip with an auto-focus function. Background technique [0002] At present, microfluidic chips are widely used in various fields, including medical diagnosis, environmental protection, drug screening, gene sequencing, etc., for high-speed and efficient separation or processing of biological macromolecules such as DNA, peptides, and proteins. Fluorescence detection is one of the important means to determine the composition and content of related components after the microfluidic chip has been systematically processed. [0003] The basic principle of the existing microfluidic chip fluorescence detection optical device is: the laser light generated by the laser passes through a filter to become approximately monochromatic light; the monochromatic light is irradiated into the micropipe of the chip through t...

AI Technical Summary

Problems solved by technology

In order to adapt to different types of chips, the micropipe positioning of the existing microfluidic chip fluorescence detector adopts a manual method, and the chip is moved manually, which requires the operator to have certain experience and takes a long time;

[0006] (2) The laser wavelength used for excitation light is fixed at one wavelength and cannot be freely replaced, which limits the choice of fluorescent dyes;

[0007] (3) The position of the objective lens group in the optical path system is fixed, and the working distance of the objective lens group cannot be adjusted according to the wavelength of the excitation light, which limits the sensitivity of the detection;

[0008] (4) The size of the aperture is fixed, and the size of the aperture cannot be adjusted according to the needs, which limits the sensitivity of the detection

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