Method for obtaining plant with yellow seedlings in red pepper seedling stage
A pepper seedling stage technology, applied in the field of breeding, can solve the problems that restrict the wide application of pepper hybrids, increase investment, and have false hybrids.
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[0018] According to the technical solution of the present invention, the specific steps are as follows:
[0019] A. The dose of dry pepper seeds is 70 (1×10Gy), and the dose rate is 1.6063Gy / min 60 After Co-γ-ray irradiation, soak the seeds in warm water at (28±1)°C for 6 hours.
[0020] B. Use 0.05mol / L, HNO of pH=4.5 with the capsicum seeds after the above-mentioned step treatment 2 The solution was shaken and soaked for 60 minutes under the condition of 28°C±1°C, and then washed with tap water for 60 minutes at room temperature to terminate its medicinal effect, and then the mutagenized capsicum seeds could be obtained.
[0021] C. Sow the capsicum seeds of mutagenesis treatment on the seedbed, obtain the capsicum mutant plant (M 1 Generation), when the plants grow to 10-12 true leaves, they are planted in greenhouses with a row spacing of 40×20 cm, and the management in the field is the same as in the field.
[0022] m 1 M 1 Generation selfing seed——M 2 .
[0023] m...
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