Sewage and sludge fermentation process of producing biopesticide bacillus thuringiensis

A Bacillus thuringiensis and sewage sludge technology, applied in the field of environmental biology, can solve the problems of restricting the promotion of Bacillus thuringiensis preparations and high production costs, and achieve the effects of less content, environmental protection and large sludge volume.

Inactive Publication Date: 2006-05-17
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the production of Bacillus thuringiensis preparations mostly uses high-priced agricultural and sideline products such as soybean powder, starch, and yeast extract as raw materials, and the production cost is relatively high, which limits the further promotion of Ba

Method used

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  • Sewage and sludge fermentation process of producing biopesticide bacillus thuringiensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Sampling

[0020] The remaining activated sludge of Nanjing Suojin Village Sewage Treatment Plant (treatment of domestic sewage), the basic properties of the sludge are shown in Table 1 and Table 2.

[0021] Solid content

%

organic matter

%

All N

g.kg -1

NH 4 + -N

mg.L -1

Water soluble P

mg.L -1

Water-soluble Mg 2+

mg.L -1

Water soluble K +

mg.L -1

2.1

62.5

45.2

86.3

131

11.5

546

[0022] Total Cr

Total Cd

Total Ni

Total Pb

Total Zn

Total Cu

58.3

2.45

22.2

56.2

450.3

170.7

[0023] (2) Expanded cultivation of B.t seeds: Take a ring of B.t 546 bacterial lawn from the inclined surface and inoculate it into 100mL sterile bacterial liquid medium (g / L) (beef extract 5, peptone 10, NaCl 5, pH=7.2) , placed on a shaker at 28°C with a rotation speed of 180r / m for ...

Embodiment 2

[0035] (1) Sampling

[0036] The remaining sludge of Suzhou Chengxi Sewage Treatment Plant (treatment of domestic sewage), the properties of the sludge are shown in Table 3 and Table 4.

[0037] Solid content

%

organic matter

%

All N

g.kg -1

NH 4 + -N

mg.L -1

Water-soluble PO 4 3-

mg.L -1

Water-soluble Mg 2+

mg.L -1

Water-soluble K +

mg.L -1

4.2

43.87

38.4

82.6

124.3

10.5

200

[0038] Total Cr

Total Cd

Total Ni

Total Pb

Total Zn

Total Cu

29.3

1.30

22.2

85.7

117.8

153.4

[0039] (2) Expanded cultivation of B.t seeds: Take a ring of B.t 546 bacterial lawn from the inclined surface and inoculate it into 100mL sterile bacterial liquid medium (g / L) (beef extract 5, peptone 10, NaCl 5, pH=7.2) , placed on a shaker at 28°C with a rotation speed of 180r / m for 12h.

[0040...

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Abstract

The present invention relates to technology of utilizing waste in producing high efficiency biopesticide, sewage sludge with solid content of 1.5-5 % is used as the material for fermentation producing Bacillus thuringiensis (B.t). Sludge is filled into fermenting tank, pH regulated to 7.2, sterilized at 121 deg.c for 20 min and inoculated with B.t seed in 1-4 wt%. After aerobic fermentation at 28 deg.c and 200 rpm stirring for 30 hr, the fermented matter with cell number up to 10E9 and spore accounting for over 90 % is discharged. Sludge with rich organic carbon, nitrogen and inorganic salt is excellent medium for B.t to grow, and compared with culturing in soybean powder culture medium, the sewage sludge fermentation process has 10 hr shorter production period, higher biotoxicity of crystal cell mixture and 3 times lower cost.

Description

1. Technical field [0001] The invention relates to a method for producing bio-pesticide Bacillus thuringiensis by fermenting sewage sludge, which is a technology for producing high-efficiency bio-pesticide by using waste, and belongs to the field of environmental biotechnology. 2. Technical background [0002] Most sewage treatment plants in my country use the activated sludge method for sewage purification, and produce sewage sludge accounting for about 0.3-0.7% of sewage inflow while purifying sewage. The lack of suitable disposal outlets for these increasingly large and highly concentrated wastes has become one of the important factors that seriously plague the healthy development of my country's sewage treatment industry. [0003] On the other hand, the current use of chemical pesticides to control plant diseases and insect pests plays an important role in increasing agricultural production, but the residues of chemical pesticides are very harmful to the environment and ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02
Inventor 周立祥王世梅
Owner NANJING AGRICULTURAL UNIVERSITY
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