Saflor yellow pigment rich in saflor yellow pigment B, its preparation method and use
A technology of safflower yellow and safflower, which is applied in the field of safflower yellow and its preparation, can solve the problems that the preparation method and application have not been reported yet, and achieve the effects of low cost, high separation efficiency and increased utilization times
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Embodiment 1
[0031] Take 20kg of safflower medicinal material, add 8 times the amount of water, heat and decoct for 2 hours, then filter, take the filtrate and concentrate to a density of 1.15; add ethanol to the concentrated solution until the alcohol concentration is 80%, alcohol precipitation at room temperature or 4°C for 24 hours, centrifuge to remove the precipitate, take the supernatant, evaporate the ethanol under reduced pressure until there is no alcohol smell; add 5 times of water to the concentrated solution, precipitate at 4°C for 24 hours, and centrifuge to remove the precipitate; take the above supernatant on DM- 130 macroporous resin, eluted with 15% ethanol for 5 column volumes, discarded; continued to elute with 50% ethanol for 8 column volumes; Elute 5 column volumes, discard, and then elute 5 column volumes with 95% ethanol solution, collect the eluate, concentrate until it has no alcohol smell, and dry to obtain safflower yellow, which is measured by HPLC. The content ...
Embodiment 2
[0033] Take 20 kg of safflower medicinal material, add 15 times the amount of water to soak for 24 hours, then filter, take the filtrate and concentrate to a density of 1.05; add ethanol to the concentrated solution until the alcohol concentration is 80%, alcohol precipitation at room temperature or 4°C for 24 hours, Remove the precipitate by centrifugation, take the supernatant, evaporate ethanol under reduced pressure until there is no alcohol smell; add 8 times of water to the concentrated solution, precipitate at 4°C for 24 hours, and remove the precipitate by centrifugation; take the above supernatant and put it on AB-8 large Pore resin, elute with 5% ethanol for 8 column volumes, discard; continue to elute with 30% ethanol for 3 column volumes; collect the above eluate and load it on the treated polyamide column, and first elute with 60% ethanol 3 column volumes were discarded, and 8 column volumes were eluted with 95% ethanol solution, and the eluate was collected and ...
Embodiment 3
[0035] Take 20kg of safflower medicinal material, add 12 times the amount of water to soak for 12 hours, then filter, take the filtrate and concentrate to a density of 1.10; add ethanol to the concentrated solution until the alcohol concentration is 80%, alcohol precipitation at room temperature or 4°C for 12 hours, Remove the precipitate by centrifugation, take the supernatant, evaporate ethanol under reduced pressure until there is no alcohol smell; add 3 times water to the concentrated solution, precipitate at 4°C for 24 hours, and remove the precipitate by centrifugation; take the above supernatant and put it on D-101 large Pore resin, eluted with 15% ethanol for 3 column volumes, discarded; continue to elute with 50% ethanol for 8 column volumes; collect the above eluate and load it on the treated polyamide column, and first elute with 60% ethanol 8 column volumes, discarded, and then eluted with 80% ethanol solution for 5 column volumes, collected the eluate, concentrat...
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