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Alsophila gigantea var. gigantea breeding method

A technology of cypress and spores is applied in a breeding field of black cypress, which can solve the problems of resource destruction of wild black cypress, lagging artificial propagation and cultivation technology, sporadic distribution, etc., so as to reduce the destruction of wild resources and environment, The effect of meeting the needs and wide application prospects

Inactive Publication Date: 2006-06-21
INST OF BOTANY CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has strict requirements on the habitat, its distribution is relatively sporadic, and its population is generally small. However, due to its high ornamental and medicinal value, the market demand for it is relatively large. As a result, the resources of wild Cymbidium macrophyllum have been severely damaged, and it has now become an endangered fern
[0004] At present, Cyathea dreei (Finnie & van Staden.Multiplication of the tree fern Cyathea dreei.HortScience, 1987, 22(4): 665), Alsophilaspinulosa (Hook.) Tryon) (Bi Shirong, Su Chengduan, Xu Zhenglan, He Liming, Research on the tissue culture of C. spinosa, Communications of Plant Physiology, 1985, (1): 38; Cheng Zhiying, Zhang Fenglei, Lan Qinying, Xu Zaifu, Tao Guoda, Research on the rapid propagation and germplasm preservation technology of C. spinosa, Yunnan Plant Research, 1991 , 13(2): 181-188) and the tissue culture method of Alsophila costularis Bak. (Cheng Zhiying, Liu Daohua, Tissue Culture of Alsophila costularis Bak., Plant Physiology Communications, 1992, 28(3): 210-211), However, there is no report on the propagation method of C.

Method used

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  • Alsophila gigantea var. gigantea breeding method
  • Alsophila gigantea var. gigantea breeding method
  • Alsophila gigantea var. gigantea breeding method

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Experimental program
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Embodiment 1

[0019] The spore culture of embodiment 1, Cymbidium macrophyllum

[0020] Medium:

[0021] 1 / 10 MS solid medium: the macro and trace elements are 1 / 10 of the full amount of MS medium, the content of iron salt and organic matter is the same as that of MS medium, 0.5% (W / V) agar, pH5.8. After boiling, it was divided into 100mL Erlenmeyer flasks, and then sterilized by high-pressure steam at 121°C for 20min.

[0022] 1. Sterilization of the spores of Cymbidium macrophyllum

[0023] Take 5mg of mature spinosa spores in a 1.5mL centrifuge tube, drop them into sterile water, oscillate fully to make a suspension, let stand for 2 hours, centrifuge at 4000r / min for 1min, discard the supernatant; then drop into the centrifuge tube Add about 1.2mL of 5% NaOCl solution to sterilize the spores, centrifuge at 4000r / min for 1min after 4min, discard the supernatant; finally rinse with sterile water 4-5 times to obtain a sterile spore suspension.

[0024] 2. Inoculation and cultivation of s...

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Abstract

The present invention discloses Gymnosphaera gigantea propagating process. Gymnosphaera gigantea spore is first sterilized and then inoculated onto1 / 12-1 / 4MS solid culture medium to obtain sporophore seedling, and the sporophore seedling is transplanted to obtain Gymnosphaera gigantea. The present invention provides one way of propagating Gymnosphaera gigantea artificially. The said process makes it possible to obtain great amount of sporophore with small amount of spore, and realize the large scale industrial production of Gymnosphaera gigantea. In addition, the present invention provides excellent experiment system for relevant research and has wide application foreground.

Description

technical field [0001] The invention relates to a method for propagating plants, in particular to a method for propagating Cymphila spinulosa. Background technique [0002] There are more than 500 species of 6 genera and 500 species of Cyatheaceae plants in the world, which are produced in tropical and subtropical mountains. my country is located in the northern edge of the distribution area of ​​Cyphilaceae plants, and there are not many species. Currently, only 14 species and 2 varieties are known, mainly distributed in Southwest and South China. The vast majority of species of Cyphilaceae plants have tree-like upright stems in the shape of trees, and large pinnate leaves are clustered on the top of the stems to form umbrellas, so they are also called tree ferns, and they are called "living fossils" in the plant kingdom ". This family of plants not only has high ornamental value in gardens, but also can be used medicinally. Its rhizomes have the functions of clearing awa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 石雷徐艳
Owner INST OF BOTANY CHINESE ACAD OF SCI
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