Antrodia camphorata mycelium fermented extract and application thereof

A technology of Antrodia camphorata fungi and extracts, which is applied in the field of Antrodia camphorata mycelium fermentation extracts, and can solve problems such as carrying improved varieties and unseen products

Inactive Publication Date: 2006-07-12
LAIYANG AGRI COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Some progress has been made recently in the study of the crude extract of Antrodia camphorata mycelium or its polysaccharides in pharmacology or biological activity. Chinese patent 01123613.2 "Solid culture method of Antrodia camphorata, obtained solid culture and its products and uses" ; 200410008046.4 "New mixtures and compound...

Method used

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  • Antrodia camphorata mycelium fermented extract and application thereof
  • Antrodia camphorata mycelium fermented extract and application thereof
  • Antrodia camphorata mycelium fermented extract and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Antrodia camphorata (Antrodia camphorata) is separated from the Antrodia camphorata fruiting body, and sent to the Dongfanghong Space Center of the Ministry of Aeronautics and Astronautics of the People’s Republic of China to carry it on a satellite. 5~28℃, microgravity level 10 -3 ~10 -5 , the perigee altitude is 200-210 kilometers, the apogee height is 300-400 kilometers, the orbit period is 90 minutes, and the spaceflight is 18 days, and the satellite is carried for mutagenesis.

[0039] After the strains of Antrodia camphorata were mutagenized by satellite, they were activated and cultured several times by conventional methods, the morphology was observed, and the differences between the strains before and after the mutagenesis were compared.

[0040] ① Mycelium Morphological Observation

[0041] After the mycelium of Antrodia camphorata is cultured on the plate, it can be observed through light microscope and environmental scanning electron microscope that there ...

Embodiment 2

[0057] Embodiment 2: Comparison of the strain culture characteristics before and after carrying Antrodia camphorata

[0058] (1) Temperature comparison test

[0059] The results of the analysis test showed that the germination temperature range of mycelium of Antrodia camphorata Ac001 was 6-36°C, which was wider than that of the starting strain (10-32°C); the suitable temperature range of mycelium of Antrodia camphorata Ac001 was 24°C-32°C. °C (colonies are orange-red), and the suitable culture temperature for the starting strain is 28-30°C (colonies are orange-red).

[0060] (2) pH comparison test

[0061] It is found through experiments that when the initial pH is in the range of pH3-pH12, the mycelium of Antrodia camphorata Ac001 can grow; the pH range suitable for the growth of mycelium of Antrodia camphorata is pH5-7; when the pH=7, the culture properties are good .

[0062] While the initial pH of the starting strain was at pH4 and pH10, the mycelium growth was very w...

Embodiment 3

[0069] Embodiment 3: Preparation of Antrodia camphorata mycelium fermentation extract

[0070] (1) Take the whole solution of Antrodia camphorata mycelium fermentation (the filtrate of mycelium and fermentation liquid), and concentrate it under reduced pressure in a conventional manner, so that its volume is concentrated to 1 / 4 of the original volume;

[0071] (2) extracting the above-mentioned concentrated fermentation broth with 70% ethanol by volume percentage, wherein the amount of ethanol added is 4 times the volume of the concentrated solution;

[0072] (3) Heating for 1 hour at 70°C;

[0073] (4) Separation is carried out by conventional methods, and impurities are removed by secondary filtration, and the ethanol extract is obtained by separation;

[0074] (5) Concentrate the above-mentioned ethanol extract under reduced pressure in a conventional manner, so that its volume is concentrated to 1 / 5 of the original volume;

[0075] (6) Precipitate the concentrated ethano...

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Abstract

The invention discloses an Antrodia camphorate mycelium fermentation extract abstracted by ethanol, wherein the Antrodia camphorata bacterial strain Ac001 was preserved in the China General Microbiological Culture Collection Center with a docket number of CGMCC No.1460. The Antrodia camphorate mycelium fermentation extract has appreciable actions in inhibiting hepatitis B virus HbsAg, e antigen HbeAg and HBV DNA secretion and resisting cancers especially liver cancer.

Description

technical field [0001] The invention relates to an antrodia camphorata mycelium fermented extract and application thereof. Background technique [0002] Antrodia camphorata is also called camphor mushroom. It generally grows on the inner wall of the hollow wood of the small-leaved sassafras tree, bell-shaped; the hymenium is orange-red and bitter in taste. The mycelium of the new species of Antrodia camphorata is orange-red and flesh-pink after cultivation. Microscopically, the hyphae are slender, septate, lock-shaped, and have a large number of conidia. The conidia are orange-red, flesh-pink or yellow due to different culture conditions. [0003] Some progress has been made recently in the study of the crude extract of Antrodia camphorata mycelium or its polysaccharides in pharmacology or biological activity. Chinese patent 01123613.2 "Solid culture method of Antrodia camphorata, obtained solid culture and its products and uses" ; 200410008046.4 "New mixtures and compoun...

Claims

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Application Information

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IPC IPC(8): A61K36/06A61P35/00C12N1/14
Inventor 宋爱荣赵晨张丽郭立忠黄芳
Owner LAIYANG AGRI COLLEGE
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