Check patentability & draft patents in minutes with Patsnap Eureka AI!

Promotion of nerve cell differentiation by human14-3-3 yupsilon protein

A technology of nerve cells and proteins, which can be applied in the fields of nervous system diseases, genetic material components, biochemical equipment and methods, etc., and can solve many problems.

Inactive Publication Date: 2006-07-19
THE INST OF BASIC MEDICAL SCI OF CHINESE ACAD OF MEDICAL SCI
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] There are not many reports about the role of 14-3-3 protein in nerve cell differentiation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Promotion of nerve cell differentiation by human14-3-3 yupsilon protein
  • Promotion of nerve cell differentiation by human14-3-3 yupsilon protein
  • Promotion of nerve cell differentiation by human14-3-3 yupsilon protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0040] 2. The overexpression of human 14-3-3ε promotes the differentiation of nerve cells

[0041] We used the primers GFP-epsilon FX and GFP-epsilon RE to amplify the human 14-3-3εcDNA recombinant plasmid. The PCR product was subjected to EcoR I and Xho I double enzyme digestion, and the pEGFP plasmid was subjected to double enzyme digestion. The vector and The fragments were recombined and ligated, followed by transformation of DH5α. The recombinant plasmid was identified as a positive plasmid by XhoI / EcoR I digestion, and the sequencing results showed that the insertion direction and reading frame of the 14-3-3ε coding region were correct.

[0042] We used Fugene 6 to transfect cultured rat neural stem cells with pEGFP and pEGFP-14-3-3ε respectively, and observed the morphological changes of neural stem cells after 48 hours. The result is as figure 1 As shown, the morphology of neural stem cells overexpressing pEGFP did not change ( figure 1 a), neural stem cells ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Login to View More

Abstract

The invention relates to the human 14-3-3ªŠalbumen that over expresses in rat nerve stem cell and can promote differentiation of nerve cell. Wherein, with human 14-3-3ªŠas bait albumen, screening cDNA library human fetal brain cDNA library with yeast di-hybridization technique to obtain an interactive albumen Math2. The albumen external association experiments have proved that Math2 can interact with 14-3-3ªŠspecially; both the CIFLA and luciferase report genetic analysis show human 14-3-3ªŠ interacts with Math2 to enhance its nucleus location and transcription activation effect of GAP-43 promotor mediated by Math2. The results disclose the molecular mechanism to provide molecular target for opposite drug exploitation.

Description

Technical field: [0001] The present invention uses mammalian cell transfection technology to discover that human 14-3-3ε protein is overexpressed in rat neural stem cells to promote the differentiation of nerve cells, and uses yeast two-hybrid and co-transformation, cell immunofluorescence and other technologies to discover and confirm the human 14-3-3ε protein 14-3-3ε protein can interact with Math2 protein, and this interaction promotes the nuclear localization of Math2, and the interaction between human 14-3-3ε protein and Math2 protein is enhanced by double luciferase transcriptional activity experiments Math2 protein mediates the transcriptional activation of GAP-43 and up-regulates the expression of GAP-43, thus revealing the molecular mechanism of human 14-3-3ε protein promoting nerve cell differentiation, and providing a possible molecular target for the development of drugs for the treatment of neurodegenerative diseases. [0002] The invention relates to the field of...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/87C12N5/00C12N5/08C12Q1/68A61K48/00A61P25/00
Inventor 王来元彭小忠刘奔韩春雨强伯勤袁建刚
Owner THE INST OF BASIC MEDICAL SCI OF CHINESE ACAD OF MEDICAL SCI
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com