Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Rice cell cycle protein dependent kinase inhibitor gene

A kinase-dependent and cell cycle-dependent technology, applied in the field of cyclin-dependent kinase inhibitor genes in rice, can solve the problem of rare research reports on cyclin-dependent kinase inhibitors

Inactive Publication Date: 2006-07-26
XIAMEN UNIV
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a new functional gene with cell cycle regulation cloned by RT-PCR to solve the problem that the current research on cyclin-dependent kinase inhibitors in rice is rare, that is, rice cyclin-dependent kinase inhibitors The first cyclin-dependent kinase inhibitor gene, named OsICK1 gene

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Rice cell cycle protein dependent kinase inhibitor gene
  • Rice cell cycle protein dependent kinase inhibitor gene
  • Rice cell cycle protein dependent kinase inhibitor gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Using the total RNA of rice variety 9311 as a template and based on the nucleic acid sequence published on NCBI, two pairs of primers were designed for PCR amplification. The amplified product was recovered, and 7 μl of the recovered product was ligated with 1 μl of T-vector overnight at 16°C. The ligated product was transformed into Escherichia coli competent cells, and recombinants were selected for identification by PCR and enzyme digestion.

[0048] The positive recombinant pMD-18T-OsICK1 was picked and sent to Boya Company for sequencing.

Embodiment 2

[0050] The nucleic acid sequences at both ends of the OsICK1 gene were selected, two pairs of primers were synthesized, and EcoR I and BamH I restriction sites were respectively introduced into the primers. Then pMD-18T-OsICK1 was used as a template for PCR amplification. The PCR amplification conditions were pre-denaturation at 94°C for 5 min; denaturation at 94°C for 45 sec, annealing at 55°C for 1 min, and 1 min at 72°C for 36 cycles; extension at 72°C for 10 min. Recover the PCR product, and EcoR I and BamH I double enzyme digestion respectively, recover the digestion product. The intermediate vector pBpF was double-digested with EcoRI and BamH I, and the digested product was recovered. Take 1μlT 4 Ligase Buffer, 6 μl recovered EcoR I and BamH I double-digested PCR product, 2 μl EcoR I and BamH I double-digested pBpF product, 10 U T 4 Ligase, ligated overnight at 16°C, transformed the ligated product into Escherichia coli competent cells, selected recombinants, performed...

Embodiment 3

[0053] Preparation of co-culture medium: Pick glycerol bacteria stored at -20°C in 1.5ml LB (containing Kan 50μg / ml), shake at 28°C for 32h, then pipette 150μl of Agrobacterium liquid into 10ml LB (containing Kan 50μg / ml) After culturing at 28°C for 24 hours, centrifuge to collect the cells, suspend the cells with an equal volume of AAM liquid medium, and add acetosyringone (AS) to the cells to make the concentration reach 100 μmol / L.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

This invention relates to a rice cell cycle protein dependently kinases inhibitor gene, named OsICK1. This invention provides a RT-PCR method for producing a new function gene that can control the cell cycle, that is the first cell cycle protein dependently kinases inhibitor gene OsICK1 gene. The length of the gene coding list is 585bp, it codes 194 amino acids, wherein 1-3 ATG is the starting codons, and 583-585 TGA is the final codons. The clone method as follows: analyzing the result according to blast, designing inducer based by the nucleic acid sequence that is published on NCBI, extracting rice gross RNA, expanding 585bp nuclei acid snippet and recollecting them to connect with the pMD-18T carrier to measuring and corroborating.

Description

technical field [0001] The invention relates to the first cyclin-dependent kinase inhibitor (cyclin-dependent kinase inhibitor) gene in rice, which is named OsICK1. Background technique [0002] The cell cycle is the basic process of cell life activities. In eukaryotes, cyclin-dependent kinase (CDK) is the central component of cell cycle regulation (Morgan. play a central regulatory role. Cyclin-dependent kinase (CDK) can combine with cyclin to form Cyclin-CDK complex, activate the kinase activity of CDK, phosphorylate substrate protein, and regulate the operation of cell cycle. Different types of CDKs can combine with different types of cyclins to form different CDK kinases, which exhibit activity in different phases of the cell cycle and regulate different phases of the cell cycle. [0003] Cyclin-dependent kinase inhibitor (ICK) plays an important regulatory role in cell cycle progression. ICK negatively regulates cell cycle progression by inhibiting CDK activity at a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/10C12N15/67C12N15/70C12N15/74C12N15/82
Inventor 陈亮何艺宾沈明山张红心曾雅明王蕾
Owner XIAMEN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com