Safety high-efficient continuous enclosed type cell culture and virus production-inactivation system

A cell culture and closed technology, applied in the system field of bioengineering technology, can solve the problems of unfavorable large-scale, continuous, safe production of virus vaccines, non-closed, etc., and achieve the effect of increasing virus production and reducing impact

Active Publication Date: 2006-10-04
上海丽坤生物科技股份有限公司
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  • Abstract
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Problems solved by technology

In the above two systems, the two processes of cell culture and virus proliferation have continuous production separately, but the whole process is intermittent

Method used

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  • Safety high-efficient continuous enclosed type cell culture and virus production-inactivation system
  • Safety high-efficient continuous enclosed type cell culture and virus production-inactivation system
  • Safety high-efficient continuous enclosed type cell culture and virus production-inactivation system

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Embodiment Construction

[0030] Such as figure 1As shown, the present invention includes: gas supply unit 1, steam generator 2, cell growth medium supply device 3, cell expansion unit 4, primary perfusion collection device 5, basal medium supply device 6, virus proliferation unit 7, cell / Carrier separation device 8, virus collection device 9, heat source supply device 10, acid / lye supply device 11, obsolete carrier collection device 12. The connection method is as follows: the gas supply unit 1 is divided into two routes, one of the outlet ports is connected to the inlet port of the cell expansion unit 4 to meet the gas requirements for cell expansion, and the other route is connected to the inlet port of the virus multiplication unit 7 to meet the requirements of the virus proliferation unit 7. Gas requirements for proliferation; the outlet port of the steam generator 2 is connected to the inlet port of the cell expansion unit 4 for in-situ sterilization of the entire system; the outlet port of the ...

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Abstract

The safe high-efficiency continual closed cell culture virus generation/deactivation system comprises: a cell amplification unit connected to a gas supplying unit, a steam generator, a supplying device for cell growth medium, a primary fill and collection device, a heat source supply device, and a acid/alkali liquid supply device; a virus amplification unit connected to a gas supplying unit, a basic medium supply device, a cell/carrier separator, a heat source supply device, and a acid/alkali liquid supply device; and a old carrier collector. This invention integrates cell and virus amplification processes for automation control and standard production with little pollution.

Description

technical field [0001] The invention relates to a system in the technical field of bioengineering, in particular to a safe and efficient continuous closed cell culture virus production / inactivation system. technical background [0002] The traditional method of culturing cells to produce viruses is to use roller bottle culture. This method has low output, it is difficult to realize automatic control, the repeatability is not good, and the batch difference is large. With the emergence of bioreactors, the above problems have been greatly solved. A general bioreactor for cell culture consists of a reactor tank, a jacket for providing heat, a gas distributor, a mechanical stirring device, and many sampling ports and sampling ports. , exhaust port, cover plate of the sensor interface, temperature sensor, pH electrode, dissolved oxygen electrode and other sensing elements, which solves the problems of mixing, mass transfer and oxygen supply well, and provides a suitable environme...

Claims

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Application Information

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IPC IPC(8): C12M3/00
Inventor 罗凤山齐瀚实陈彦田耿涛孙海英
Owner 上海丽坤生物科技股份有限公司
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