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Process of extracting total salvianolic acid and total salvianolic acid extract

A technology of salvia miltiorrhiza total phenolic acids and extracts, which is applied in the field of preparation of salvia miltiorrhiza root phenolic acids, can solve the problems of low content of salvia miltiorrhiza root phenolic acids, cumbersome operation process, and many impurities, and achieve high transfer rate, good stability, stable effect

Inactive Publication Date: 2006-11-08
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Most of the disclosed Chinese patent documents (such as CN 1534010A, CN1498615A) disclosed the extraction method of salvianolic acid, as well as the existing industrial production method, all utilize macroporous resin enrichment, the deficiency of this purification method Because the operation process is cumbersome and time-consuming, the longer the extract is in the aqueous solution state, the more serious the degradation of salvianolic acid B is, and there are more impurities (degradation products), resulting in low total phenolic acid content of salvia miltiorrhiza

Method used

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  • Process of extracting total salvianolic acid and total salvianolic acid extract

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Effect test

Embodiment 1

[0038] Take 250kg of Danshen herbal decoction pieces, soak for 30min, decoct in 8 times the amount of water at 80°C for 2 times, each time for 2h. The extracts were combined, concentrated to a relative density of 1.1 (60°C thermal measurement), adjusted to an alcohol content of 65% with 95% ethanol, and allowed to stand overnight. The filtrate was recovered with ethanol and concentrated to 1 mg / mL, acidified to pH 3.5 with 10% hydrochloric acid, filtered, the filtrate was extracted with ethyl acetate three times, the organic solvent layer was separated, concentrated under reduced pressure, and spray-dried to obtain the total phenolic acid fraction of Salvia miltiorrhiza 6.75Kg. Its total phenolic acid content is 94.1%, and salvianolic acid B content is 65.2%.

Embodiment 2

[0040] Take 250 kg of Danshen herbal decoction pieces, soak for 30 minutes, boil 8 times the amount of water twice, each time for 1.5 hours. The extracts were combined, concentrated to a relative density of 1.1 (60°C thermal measurement), adjusted to an alcohol content of 65% with 95% ethanol, and allowed to stand overnight. After recovering ethanol from the filtrate to 1 mg / mL, acidify to pH 3.5 with 10% hydrochloric acid, filter, extract the filtrate with ethyl acetate 4 times, separate the organic solvent layer, concentrate under reduced pressure, and spray dry to obtain the total phenolic acid fraction of Salvia miltiorrhiza 6.80 Kg. Its total phenolic acid content is 95.9%, and salvianolic acid B is 61.5%.

Embodiment 3

[0042] Take 250kg of Danshen herbal decoction pieces, soak for 30min, decoct in 8 times the amount of water at 80°C for 2 times, each time for 1.5h. The extracts were combined, concentrated to a relative density of 1.1 (60°C thermal measurement), adjusted to an alcohol content of 65% with 90% ethanol, and allowed to stand overnight. After recovering ethanol from the filtrate to 1 mg / mL, acidify to pH 3.5 with 10% hydrochloric acid, filter, extract the filtrate with ethyl acetate 4 times, separate the organic solvent layer, concentrate under reduced pressure, and spray dry to obtain the total phenolic acid fraction of Salvia miltiorrhiza 6.91 Kg. Its total phenolic acid content is 95.2%, and salvianolic acid B is 60.2%.

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Abstract

The process of preparing total salviaolic acid with red sage as one Chinese medicinal material and without used macroporous resin includes the following steps: decocting red sage pieces, concentrating, alcohol precipitation, acidification, ethyl acetate extraction, separating of organic solvent layer and decompression recovering of organic solvent.

Description

technical field [0001] The invention relates to a preparation method of salvia miltiorrhiza phenolic acid and a salvia miltiorrhiza total phenolic acid extract prepared by the method. Background technique [0002] Salvia miltiorrhiza is the dry root and rhizome of Salvia miltiorrhiza Bge. Bitter in nature, slightly cold; GUIXIN, Liver Channel. It has the effects of dispelling blood stasis and relieving pain, promoting blood circulation and promoting menstrual flow, clearing away heart-fire and relieving restlessness. Clinically, it is often used to treat irregular menstruation, amenorrhea, dysmenorrhea, accumulation of lumps, hepatosplenomegaly, angina pectoris and other diseases. The pharmacologically active components of Danshen are mainly divided into two categories, namely fat-soluble components and water-soluble components. Pharmacodynamic studies in recent years have proved that the water-soluble total phenolic acids of Salvia miltiorrhiz...

Claims

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Application Information

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IPC IPC(8): A61K36/537A61K31/343A61K125/00A61P9/10C07D307/84
Inventor 王强张勤崔秀明俞祥生施天慧李伟佳
Owner CHINA PHARM UNIV