Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for detecting cervical carcinoma gene

A technology for gene detection and cervical cancer, applied in the medical field, can solve the problem of inability to judge the risk of disease, and achieve the effect of low cost

Inactive Publication Date: 2006-11-08
ATTACHED OBSTETRICS & GYNECOLOGY OSPITAL MEDICALCOLLEGE ZHEJIANG UNIV
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no matter which method is used, cervical cytology screening is based on the diagnosis and screening of abnormal cell morphology that has already appeared, which belongs to secondary prevention; it cannot be judged for people who have not yet had cervical intraepithelial neoplasia (CIN) its risk
[0006] In conclusion, so far, there is no method that can detect the susceptibility of cervical cancer from the female's own genetic background and genetic characteristics

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting cervical carcinoma gene
  • Method for detecting cervical carcinoma gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0020] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0021] 1. Draw 3ml of peripheral blood from the female to be tested into an EDTA anticoagulant test tube;

[0022] 2. Primer design:

[0023] XRCC1-194P1

5'-GGGGGCTCTCTTCTTCAGGC-3'

XRCC1-194P2

5'-GGGGGCTCTCTTCTTCAGGT-3'

XRCC1-194P3

5'-CGCTGGCTGTGACTATGAAG-3'(362bp)

P21-31P1

5'-TGGACAGCGAGCAGCTGACC-3'

P21-31P2

5'-TGGACAGCGAGCAGCTGACA-3'

P21-31P3

5'-AGTCCTTCCGTGCACATGTC-3'

(397bp)

[0024] 3. DNA extraction: EDTA anticoagulated whole blood, centrifuge at 1300×g for 15 minutes, discard the plasma, absorb 400ul

[0025] 4. Transfer to a new Ep tube, add phosphate buffer solution PBS 400ul and deionized water H 2 O700ul mix, 4 ℃, 12000rpm, centrifuge for 10 minutes, discard the supernatant. Add 1000ul of PBS, mix well, centrifuge at 12000rpm at 4°C for 10 minutes, and disca...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention discloses gene detecting process of cervical carcinoma. The gene detecting process of cervical carcinoma includes collecting woman's peripheral blood or cervical cell, extracting DNA as the amplified template, determining the relevant gene information of the SNP site, designing two corresponding upstream primers and one downstream primer, adding the primers and the template for DNA amplification, taking the amplification product for electrophoresis in agarose gel containing EB, and judging the result in a ultraviolet spectrometric projector or gel image analyzing scan system. The present invention has the features of being simple, practical, low in cost and suitable for being spread.

Description

technical field [0001] The invention relates to the medical field, in particular to a genetic detection method for cervical cancer genetic risk. Background technique [0002] Cervical cancer is one of the three most common malignant tumors in women today, and it has been on the rise in recent years, seriously threatening the health of women. Over the years, research on the etiology and pathogenesis of cervical cancer has made some progress, especially in the study of external causes. The most significant discovery is the close relationship between HPV infection and cervical cancer. A large number of studies have shown that the persistent infection of high-risk HPV (such as HPV16, HPV18, etc.) is necessary for the carcinogenesis of cervical epithelial cells, but only a small part of HPV infection Those who develop cervical cancer eventually show that HPV infection alone is not enough to cause tumors; the genetic background of the host is the key factor for tumors to occur af...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68
Inventor 陈怀增叶枫谢幸黄金阳田其芳
Owner ATTACHED OBSTETRICS & GYNECOLOGY OSPITAL MEDICALCOLLEGE ZHEJIANG UNIV