Method for preparing natural anthraquinone pigments using bamboo parasitic fungus fermentation

An anthraquinone pigment and bamboo yellow fungus technology, which is applied in the field of anthraquinone pigments and natural anthraquinone pigments, can solve problems such as production restrictions, and achieve the effect of high safety

Inactive Publication Date: 2006-12-27
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complexity of biosynthesis and metabolism, many natural pigments are difficult to chemically synthesize under artificial control, and the production is greatly restricted.

Method used

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  • Method for preparing natural anthraquinone pigments using bamboo parasitic fungus fermentation
  • Method for preparing natural anthraquinone pigments using bamboo parasitic fungus fermentation
  • Method for preparing natural anthraquinone pigments using bamboo parasitic fungus fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] The preparation of embodiment 1 bamboo yellow bacteria fermented liquid

[0058] 1. Inoculate the mycelia of Bambusicola p. hennigs into the fresh slant culture medium, and the strain of Shiraiabambusicola P. Hennigs LBR-SB6 was purchased from the Culture Collection Center of the Institute of Mycoplasma, Jilin Agricultural University. As a known strain, its isolation and identification can refer to the relevant literature mentioned above, such as Wei Jingchao, "Handbook of Fungal Identification", Shanghai Science and Technology Press, Shanghai, published in 1979, p238, etc. The culture temperature is 25° C., and the slant medium formula is (in g / L): 25 glucose, 200 potato, and 20 agar.

[0059] After the mycelia covered the slant, the slant strains were inserted into 250 mL Erlenmeyer flasks (5 bottles in total) containing 75 mL of shaker culture medium, and cultured on a shaker at 25.5° C. and 150 rpm for 130 hours. Wherein the formula of the shake flask culture mediu...

Embodiment 2

[0064] The preparation of embodiment 2 formula (I) pigment

[0065] 1. The fermented liquid obtained in embodiment 1 is centrifuged at 3000 rpm, the supernatant obtained.

[0066] 2. Concentrate 100 mL of the above fermentation supernatant to about 46 mL with a rotary evaporator under reduced pressure to obtain a fermentation concentrate. Adjust the pH of the concentrated solution to about 3.0 with 6 mol / L HCl, add an equal volume of chloroform for extraction, filter the chloroform extract with suction, and remove the milky substance to obtain about 42 mL of clear liquid, which is evaporated under reduced pressure to about 2 mL.

[0067] 3. Purification by Column Chromatography

[0068] (1) Silica gel column chromatography

[0069] Chromatographic column specifications: glass column; column length × inner diameter: 30mm × 20mm; packing height: 20mm; column volume: 400mm

[0070] Packing specifications: column chromatography silica gel, coarse pore (ZCX-II) 200-300 mesh, produ...

Embodiment 3

[0090] The preparation of embodiment 3 formula (I) pigment

[0091] The bacterial classification used in this embodiment and slant culture medium are identical with embodiment 1.

[0092] 1. Insert the mycelia of Bamboo Flask into the fresh slant culture medium, culture at 25°C, after the mycelium grows all over the slant, put the slant bacteria into a 250mL Erlenmeyer flask with 75mL shake flask medium (a total of 3 bottles), cultivated for 140 hours at 22°C and 120 rpm in a shaker;

[0093] 2. Insert the above shake flask strains into 500mL Erlenmeyer flasks equipped with 150mL expansion medium (10 flasks in total), the inoculum amount is to insert 15mL shake flask seeds into each 500mL Erlenmeyer flask, and shake at 22°C and 120 rpm. Bed culture for 96 hours;

[0094] 3. Put 1500mL of the above-mentioned expanded cultured bacteria into a 50L seed tank equipped with 28.5L primary seed medium for cultivation. The total amount of fermentation liquid in the seed tank is 30L; ...

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Abstract

The invention relates the method for making anthraquinone pigments with bamboo yellow bacterium submerged cultuse, submerged cultuse liquid and anthraquinone pigments 1, 5- dihydroxy-3- methoxy-7- methyl-anthraquinonyl. The method comprises the following steps: using bamboo yellow bacterium as strain, carrying out fluid culture, fluid enlargement culture, one-level seed culture and submerged cultuse culture, separating the fermentation liquor, purifying, and getting the products. The invention first produces the anthraquinone pigments (I), which is brown solid. The products can be used as food additives, and have oxidation resistance and bacteriostasis.

Description

technical field [0001] The present invention relates to the field of biological fermentation, specifically, the present invention relates to the liquid submerged fermentation technology of bamboo yellow fungus, and the natural anthraquinone pigment 1,5-dihydroxy-3 prepared by applying the fermentation technology to fermented liquid and formula (I) -methoxy-7-methylanthraquinone method. The present invention also relates to the fermented broth of bamboo yellow fungus and the anthraquinone pigment of formula (I) prepared by the process. [0002] The invention firstly realizes the preparation of natural pigment by the bamboo yellow fungus fermentation technology, which is of great significance for the industrial development of bamboo yellow. Background technique [0003] Pigments are also called colorants, and food additives whose main purpose is food coloring are called colorants. Due to different sources, it is generally divided into two categories: natural pigments and syn...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09B1/02C09B1/14C12P7/26
Inventor 石贵阳丁重阳张梁蔡宇杰楼志华
Owner JIANGNAN UNIV
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