Method for fixing enzyme by biological organic composite membrane

Abstract

The invention guides in one biological macromolecule layer (gel layer) between the inorganic film and base electrode, to form biological inorganic composite film to fix the enzyme, wherein when the inorganic sol liquid is coated on the biological base gel layer, since the gel has some flexibility, the gel skeleton can obtain buffer when contracts, to avoid crack; and since the gel and base electrode have better adhesive function, the separation of enzyme film can be avoided. The invention can has better enzyme fixing effect.

Description

technical field [0001] The invention relates to a method for immobilizing enzymes in the process of preparing biosensors, more specifically to a method for immobilizing enzymes with bio-inorganic composite membranes. Background technique [0002] Enzyme immobilization is a key step in the preparation of biosensors. Since the mid-1990s, the inorganic membranes prepared by the sol-gel method have been applied to the immobilization of enzymes. The method uses an inorganic silicate porous material as an enzyme carrier, and the inorganic membrane has the advantages of good chemical inertness, physical rigidity, almost no swelling in solution, adjustable pore size, and optical transparency. However, the application of this method in biosensors is limited due to the fact that the gel is easy to crack during the film formation process and is not firmly combined with the substrate electrode. Many scholars have been committed to solving the above problems, for example, by adding sui...

AI Technical Summary

Problems solved by technology

The invention will effectively overcome the disadvantages that the gel is easy to crack during the film forming process and is not firmly combined with the substrate electrode, and obtains a good enzyme immobilization effect