Ocean salt monad and its using method in biological flocculation and heavy metal adsorption

A technology of halomonas and biological flocculants, applied in the field of microbial resources and environmental biology, to achieve the effects of high viscosity, low cost and simple extraction process

Inactive Publication Date: 2007-01-24
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, trace amounts of heavy

Method used

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  • Ocean salt monad and its using method in biological flocculation and heavy metal adsorption
  • Ocean salt monad and its using method in biological flocculation and heavy metal adsorption
  • Ocean salt monad and its using method in biological flocculation and heavy metal adsorption

Examples

Experimental program
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Effect test

Embodiment 1

[0017] Extraction of exopolysaccharides

[0018] The cultured fermentation broth was centrifuged at 10000r / min for 20min to collect the supernatant, and the protein was removed by the savage method, and 2.5 times the volume of absolute ethanol was added, left to stand overnight at 4°C, centrifuged at 12000r / min for 10min, the precipitate was collected, vacuum-dried, and then added Redissolve in deionized water, centrifuge, precipitate with absolute ethanol, add water to dissolve, dialyze, and vacuum freeze-dry to obtain pure exopolysaccharide.

Embodiment 2

[0020] Acid-base and thermal stability of exopolysaccharide flocculated kaolin suspension

[0021] 1. The acid-base stability of exopolysaccharides in flocculation of kaolin

[0022] The exopolysaccharide extracted by the present invention can maintain high flocculation activity for kaolin suspension in a wide pH range (1-11), and all of them are above 93%, and the use concentration is only 5.0mg / L, and the dosage is relatively low , the test results see figure 1 .

[0023] 2. Exopolysaccharide exhibits thermal stability when flocculating kaolin

[0024] The exopolysaccharide extracted by the present invention was denatured at 100°C for different times, and its flocculation activity on kaolin was measured again, and it was found that it had very good thermal stability. After heating for 14 hours, it showed a significant decrease in flocculation activity. Only 5.0mg / L. See the test results figure 2 . This characteristic is several times higher than the heat ...

Embodiment 3

[0026] Adsorption effect of exopolysaccharides on various heavy metals

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Abstract

The ocean salt monad of code of V3a and separated from deep sea bottom mud of west pacific ocean may be used in preparing biological polysaccharide through a simple fermenting culture process including providing common nutritious components, glucose, ammonium chloride, phosphate, magnesium sulfate, sodium chloride and 0.06 % concentration yeast paste as the growth factors, aerating and stirring at 18-28deg.c. The polysaccharide has low cost, simple extracting process, and wide application value. It has high flocculating activity, very high heat stability, obvious flocculating effect even on high temperature and acid or alkali conditions, effect of absorbing heavy metals, especially lead and wide application foreground in sewage treatment, food production and biochemical industry.

Description

technical field [0001] The invention relates to the field of microbial resources and environmental biotechnology. Specifically, the present invention relates to the application and treatment method of Halomonas sp.V3a and its exopolysaccharide in flocculation of suspended matter and heavy metal adsorption. Background technique [0002] Polysaccharides produced by microorganisms are widespread in nature. In theory, most polysaccharides produced by bacteria have potential industrial application value. However, due to the limitations of experimental techniques and the physical properties of polysaccharides, there are only a few polysaccharides (xanthan gum, Jilan gum, etc.) are widely used in the field of biotechnology. Therefore, the market will have a huge demand for different polysaccharide products in the next ten years, and many researchers are also looking for new functional polysaccharides to meet the diversified needs of modern industry. [0003] The potential huge ap...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F1/52C12P19/04
Inventor 邵宗泽汪保江
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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