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Preparation method of puerarin extract

A production method and extract technology, applied in the field of extraction and separation of puerarin in Chinese herbal medicine, can solve the problems of high production cost, many process steps, and long production cycle, and achieve low production cost, simple process operation, and short production cycle Effect

Active Publication Date: 2007-03-14
TIANJIN TASLY MORDEN TCM RESOURCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has many process steps, long production cycle, high ratio of medicinal material to resin and a large amount of organic solvents other than ethanol, not only high production cost, but also no guarantee of safety in large-scale production, especially without the production verification of pilot scale or above. , whether it is suitable for mass production is still unknown

Method used

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  • Preparation method of puerarin extract
  • Preparation method of puerarin extract
  • Preparation method of puerarin extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Weigh 50kg Pueraria mirifica medicinal material, after crushing, add 400L water to decoct for 3 hours, then decoct with 300L water for 0.5 hour, combine the decoction liquid, cool, pass through macroporous resin D-392 with a weight of 100kg, first decoct with 3 Wash with 5 times the column volume of water, then 5 times the column volume of 60% ethanol, collect the ethanol eluent, reclaim the ethanol to obtain powder, add 4 times the amount of water, and pass through a macroporous adsorption resin with a weight of 12.5kg S-8, first eluted with 3 times column volume of water, then eluted with 6 times column volume of 20% ethanol, collected 20% ethanol eluate, recovered ethanol to obtain puerarin extract, wherein the puerarin content was 60%, The powder extraction rate was 1.9%.

Embodiment 2

[0027] Weigh 50kg powder kudzu root medicinal material, after pulverization, add 400L water to decoct for 2 hours, then decoct with 300L water for 1 hour, combine the decoction liquid, cool, pass through macroporous resin D-392 with a weight of 100kg, first decoct with 3 Elute with 5 times column volume of water, then 5 times column volume of 60% ethanol, collect the ethanol eluate, recover ethanol to obtain powder, add 4 times the amount of 1% (V / V) concentrated sulfuric acid and hydrolyze After 3 hours, adjust the pH of the hydrolyzed solution to 6.5, dilute the volume by 5 times on the basis of the hydrolyzed solution, and filter to obtain the filtrate. The filtrate passes through the macroporous adsorption resin S-8 with a weight of 12.5 kg, and washes it with water for 3 times the column volume. Then, elute with 6 times column volume of 20% ethanol, collect the 20% ethanol eluate, recover the ethanol to obtain the puerarin extract, wherein the puerarin content is 77%, and ...

Embodiment 3

[0029] Weigh 200kg powder kudzu root medicinal material, after pulverization, add 1600L water to decoct for 2 hours, then decoct with 1200L water for 1 hour, combine the decoction liquid, cool, pass through macroporous resin D-392 with a weight of 300kg, first decoct with 2 Elute with 4 times column volume of water, then 4 times column volume of 60% ethanol, collect the ethanol eluate, recover ethanol to obtain powder, add 4 times the amount of 1.5% (V / V) concentrated sulfuric acid and hydrolyze After 4 hours, adjust the pH of the hydrolyzate to 6.5, dilute the volume by 5 times on the basis of the hydrolyzate, and filter to obtain the filtrate. The filtrate passes through the macroporous adsorption resin S-8 with a weight of 50 kg, and first elutes with 3 times the column volume of water , and then eluted with 7 times column volume of 20% ethanol, collected the 20% ethanol eluate, recovered the ethanol to obtain the puerarin extract, wherein the puerarin content was 79%, and t...

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Abstract

The present invention relates to plant medicine component extracting and separating technology, and is especially a puerarin extracting and separating process from Chinese herbal material. The puerarin extracting and separating process includes the following steps: crushing root of Pueraria plant as the material, decocting, chromatographic separation with ion exchange type macroporous resin, eluting in macroporous resin column first with water and then with 20 wt% ethanol solution, collecting the eluted liquid, and recovering ethanol to obtain puerarin extract. The process has simple operation, short production period, low production cost and capacity of obtaining puerarin extract with high puerarin content, and is suitable for industrial production.

Description

technical field [0001] The invention relates to the field of extraction, separation and purification methods of plant medicinal components, in particular to the extraction and separation method of puerarin in Chinese herbal medicine. Background technique [0002] Pueraria lobata (Willd.) Ohwi is the root tuber of leguminous plants (Pueraria lobata (Willd.) Ohwi), also known as Gange, Fenge, Huanggegen, and Gemaru. Pueraria lobata has a long history of medicinal and edible use. As a commonly used traditional Chinese medicine, it is recorded in detail in the earliest pharmacy monograph "Shen Nong's Materia Medica", which is listed as a middle grade. Pueraria lobata is sweet and pungent in taste, flat in nature, and has pharmacological effects such as reducing vascular resistance, improving heart and brain blood circulation, slowing heart rate, and reducing myocardial oxygen consumption. Puerarin is the main active ingredient of traditional Chin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08
Inventor 杨悦武汤树良郑智刚刘长青陆文亮
Owner TIANJIN TASLY MORDEN TCM RESOURCES
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