Substrate for microarray of biomolecule, hybridization device, and hybridization method

Abstract

A substrate for hybridization of biomolecule, a biomolecule hybridization device, and a biomolecule hybridization method in which biomolecule is hybridized at higher rate through dielectrophoresis migration or electrophoresis by applying an AC or DC voltage to a planar electrode thereby generating an electric field, and hybridized biomolecule can be read out using a laser or the like.

Description

technical field [0001] The present invention relates to a microarray substrate for biopolymers such as DNA and RNA, a hybridization device using the substrate, and a hybridization method for increasing the hybridization speed using the device. Background technique [0002] In general, in applications such as genetic diagnosis, identification of pathogens, and detection of single nucleotide polymorphisms, hybridization techniques between probe nucleic acids and target nucleic acids are widely used in order to detect nucleic acids (target nucleic acids) to be detected. Recently, a DNA chip and a DNA microarray having a large number of probe nucleic acids immobilized on a substrate are put into practical use, and a hybridization technique is used to detect a target nucleic acid. [0003] In the manufacturing process of biopolymer microarrays (for example: DNA chips or DNA microarrays), a large number of probe DNAs must be respectively arranged and fixed on the substrate as samp...

AI Technical Summary

Problems solved by technology

In addition, hybridization requires a large amount of sample

As a result, such a long hybridization time and preparation of a large number of samples requires a lot of time, cost and labor

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