Gene detection probe and gene detection method

A gene detection and probe technology, applied in the field of biochemical analysis, can solve the problems of complex detection steps, slowness, and low hybridization efficiency, and achieve the effects of fast hybridization speed, simple and convenient operation, and high sensitivity
CN107574226BActive Publication Date: 2021-04-13广州博徕斯生物科技股份有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
广州博徕斯生物科技股份有限公司
Publication Date
2021-04-13

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Abstract

The invention discloses a gene detection probe and a gene detection method. Described gene detection probe comprises inactivation nuclease protein, the marker molecule that is connected on inactivation nuclease protein, and the guide RNA that is connected on inactivation nuclease protein; Described inactivation nuclease protein is inactivation Cas9 protein or inactivated Cpf1 protein. The gene detection method comprises incubating the gene to be detected with the gene detection probe described in claim 1 at room temperature to obtain a gene-probe complex, and by performing signal detection on the resulting gene-probe complex, Realize the genetic testing described above. The gene detection probe of the present invention can hybridize with the gene to be detected at normal temperature, has high affinity and recognition specificity for the gene to be detected, has fast hybridization speed and high sensitivity, and can realize rapid and accurate detection of the gene.
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Description

technical field

[0001] The invention belongs to the technical field of biochemical analysis, and specifically relates to a gene detection probe and a gene detection method. Background technique

[0002] Accurate, sensitive, and specific analysis of biological functional molecules (such as nucleic acids and proteins) is of great scientific significance in the fields of life science and biomedical research and clinical diagnosis of diseases. Conventional biochemical analysis and medical diagnosis have experienced the development of landmark technologies such as radiology, radioimmunology, and immuno-optics. In recent years, they have gradually transitioned to the category of molecular diagnostics. In the 21st century, thanks to multidisciplinary synthesis, nucleic acid probes and sensing technologies have achieved deeper and broader development. For example, through the development of immune-PCR (Immune-PCR) and aptamer probe technology, nucleic acid probes have been able to ...

Claims

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