Direct bilirubin detecting kit

A kit, bilirubin technology, applied in biological testing, microbial determination/inspection, color/spectral property measurement, etc. Good performance, high sensitivity, good measurement accuracy

Active Publication Date: 2007-05-23
NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing sodium nitrite oxidant kit still has limitations such as poor accuracy for low-value samples, weak anti-interference, and low sensitivity, which affects its popularization and application.

Method used

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  • Direct bilirubin detecting kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Reagent 1 (R1)

[0065] Tartaric acid-sodium hydroxide buffer (pH3.4) 7.5g

[0066] Disodium ethylenediaminetetraacetic acid (EDTA-Na 2 ) 0.2g

[0067] Thiourea ((NH 2 ) 2 CS) 1.4g

[0068] Hydroxylamine Hydrochloride 0.2g

[0069] TritonX-100 7.5ml

[0070] h 2 O to 1L

[0071] Reagent 2 (R2)

[0072] Disodium hydrogen phosphate (Na 2 HPO 4 .12H 2 O) 1g

[0073] Potassium dihydrogen phosphate (KH 2 PO 4 ) 0.25g

[0074] Sodium nitrite (NaNO 2 ) 0.7g

[0075] Disodium ethylenediaminetetraacetic acid (EDTA-Na 2 ) 2g

[0076] Sodium chloride (NaCl) 22.5g

[0077] Thimerosal (C 9 h 9 o 2 HgNaS) 0.04g

[0078] h 2 O to 1L

Embodiment 2

[0080] Reagent 1 (R1)

[0081] Tartaric acid-sodium hydroxide buffer solution (pH2.6) 37.5g

[0082] Disodium ethylenediaminetetraacetic acid (EDTA-Na 2 ) 1g

[0083] Thiourea ((NH 2 ) 2 CS) 0.1g

[0084] Hydroxylamine Hydrochloride 1.2g

[0085] Triton X-100 1.5ml

[0086] h 2 O to 1L

[0087] Reagent 2 (R2)

[0088] Disodium hydrogen phosphate (Na 2 HPO 4 12H 2 O) 5.5g

[0089] Potassium dihydrogen phosphate (KH 2 PO 4 ) 1.5g

[0090] Sodium nitrite (NaNO 2 ) 0.15g

[0091] Disodium ethylenediaminetetraacetic acid (EDTA-Na 2 ) 13g

[0092] Sodium chloride (NaCl) 4g

[0093] Thimerosal (C 9 h 9 o 2 HgNaS) 0.3g

[0094] h 2 O to 1L

Embodiment 3

[0096] Reagent 1 (R1)

[0097] Tartaric acid-sodium hydroxide buffer solution (pH2.8) 11g

[0098] Disodium ethylenediaminetetraacetic acid (EDTA-Na 2 ) 0.4g

[0099] Thiourea ((NH 2 ) 2 CS) 0.2g

[0100] Hydroxylamine Hydrochloride 0.7g

[0101] TritonX-100 2.2ml

[0102] h 2 O to 1L

[0103] Reagent 2 (R2)

[0104] Disodium hydrogen phosphate (Na 2 HPO 4 12H 2 O) 1.5g

[0105] Potassium dihydrogen phosphate (KH 2 PO 4 ) 0.4g

[0106] Sodium nitrite (NaNO 2 ) 0.4g

[0107] Disodium ethylenediaminetetraacetic acid (EDTA-Na 2 ) 3.5g

[0108] Sodium chloride (NaCl) 13.5g

[0109] Thimerosal (C 9 h 9 o 2 HgNaS) 0.07g

[0110] h 2 O to 1L

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PUM

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Abstract

The invention discloses a direct bilirubin detects reagent kit, and the kit is liquid-type double-reagent comprising reagent 1 and reagent 2. The reagent 1 is: tartaric acid-NaOH buffer solution (pH 2.6~3.4) 7.5~37.5g / L, ethylenediamine tetraacetic acid disodium 0.2~1g / L, thiourea 0.1~1.4g / L, hydroxylammonium 0.2~1.2g / L, tramadol hydrochloride 1001.5~7.5 ml / L. the reagent 2 is: Na2HPO4 1~5.5g / L, potassium dihydrogen phosphate 0.25~1.5g / L, sodium nitrite 0.15~0.7g / L, ethylenediamine tetraacetic acid disodium 2~13g / L, NaCl 4~22.5g / L, thiomersal 0.04~0.3g / L. The reagent kit of the invention has the advantages of wide linear test range, high precision and accuracy, good interference-resistance, and high sensitivity, and it has greater clinical practical value.

Description

technical field [0001] The invention relates to the technical field of biological preparations, in particular to a direct bilirubin assay kit. Background technique [0002] For the determination of bilirubin, the diazonium reagent method is traditionally used. This method has a long history and is more accurate. . Enzymatic determination of direct bilirubin has specific characteristics, but its kit is not conducive to popularization and application due to poor thermal stability of the enzyme, short storage time, and high price. [0003] At present, kits for measuring direct bilirubin by chemical oxidation methods have occupied a large share in the market, mainly including vanadic acid oxidizing kits and sodium nitrite oxidizing kits. When the vanadic acid oxidant kit measures low-value samples, the side reaction is more obvious and affects the results; when measured on an automatic biochemical analyzer, it can seriously interfere with the determination of other items; it h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/72G01N21/25C12Q1/00
Inventor 邹炳德
Owner NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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