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A kind of glycocholic acid detection kit

A technology for detecting kits and glycocholic acid, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of poor accuracy, sensitivity, and repeatability, and achieve the effects of good stability, low detection limit, and high precision

Active Publication Date: 2016-03-16
CHONGQING ZHONGYUAN BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the accuracy, sensitivity and repeatability of glycocholic acid detection kits currently on the market in my country are still poor.

Method used

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  • A kind of glycocholic acid detection kit
  • A kind of glycocholic acid detection kit
  • A kind of glycocholic acid detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] The kit of the present invention is exemplified as a double reagent, wherein:

[0064] Reagent R1:

[0065]

[0066] Reagent R2:

[0067]

[0068] Coated with mouse anti-human glycocholic acid polyclonal sensitized polystyrene latex particles, the particle diameter is 200nm, and the concentration is 1%.

[0069] Calibrator:

[0070]

[0071]

[0072] According to the required concentrations of glycocholic acid reference calibrator, the corresponding glycocholic acid standard was added to the above buffer respectively to prepare a set of calibrator concentrations of 2.5, 5.0, 10.0, 20.0, and 40.0 mg / L.

Embodiment 2

[0073] Embodiment 2: Kit usage method.

[0074] 1. Reagent preparation, the reagent is a liquid double reagent, ready to use after opening the bottle, of which:

[0075] Reagent R1:

[0076]

[0077] Reagent R2:

[0078]

[0079] Coated with mouse anti-human glycocholic acid polyclonal sensitized polystyrene latex particles, the particle diameter is 200nm, and the concentration is 1%.

[0080] Calibrator:

[0081]

[0082] According to the required concentrations of glycocholic acid reference calibrator, the corresponding glycocholic acid standard was added to the above buffer respectively to prepare a set of calibrator concentrations of 2.5, 5.0, 10.0, 20.0, and 40.0 mg / L.

[0083] 2. Automatic biochemical analyzer parameter setting

[0084] (a) Detection wavelength: the main wavelength is 600nm, and the secondary wavelength is none;

[0085] (b) Detection temperature: 37°C;

[0086] (c) Reaction time: 10 minutes, wherein, the incubation time is 5 minutes, meas...

Embodiment 3

[0104] Preparation and use of the comparison kit:

[0105] The buffer solution in the reagent R1 adopts tromethamine buffer solution 75mmol / L, pH7.2, and other reagents and experimental methods are the same as in Examples 1 and 2.

[0106] Reagent R1:

[0107]

[0108]

[0109] Reagent R2:

[0110]

[0111] Coated with mouse anti-human glycocholic acid polyclonal sensitized polystyrene latex particles, the particle diameter is 200nm, and the concentration is 1%.

[0112] Calibrator:

[0113]

[0114] According to the required concentrations of glycocholic acid reference calibrator, the corresponding glycocholic acid standard was added to the above buffer respectively to prepare a set of calibrator concentrations of 2.5, 5.0, 10.0, 20.0, and 40.0 mg / L.

[0115] The glycocholic acid detection kit prepared in Example 3 was subjected to a performance test, and the method was the same as in Example 2.

[0116] 1) Analytical sensitivity: Take a fixed-value glycocholi...

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Abstract

The invention discloses a cholyglycine detection kit and a preparation method thereof. A kit body of the kit disclosed by the invention comprises a reagent R1, a reagent R2 and a calibration product, wherein the reagent 1 comprises the following components: a biological buffer solution 1, a protective agent 1, a reaction reinforcing agent 1, a surfactant 1, a preservative 1 and a stabilizer 1; the reagent 2 comprises the following components: a biological buffer solution 2, latex particles of an anti-cholyglycine antibody, a protective agent 2, a reaction reinforcing agent 2, a surfactant 2, a preservative 2 and a stabilizer 2; and the calibration product comprises the following components: a buffer solution 3, a protective agent 3, a preservative 3 and a cholyglycine standard product. The cholyglycine detection kit disclosed by the invention adopts a dual-reagent mode, can not only effectively avoid interference of non-specific reaction, but also reduce the interference caused by turbidity of a sample to a greatest extent and ensure accurate and reliable determination result, and has the advantages of good repeatability, high precision, good stability, high detection sensitivity, wide linear test range, strong anti-interference property and the like.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a glycocholic acid detection kit and its preparation method and application. Background technique [0002] Cholyglycine (CG) is a conjugated bile acid formed by the combination of bile acid and glycine. In liver cells, cholesterol is transformed into primary bile acid through complex enzymatic reactions. Among them are cholic acid (CA) and chenodeoxycholic acid (CD-CA). There are three hydroxyl groups (C3, C7, C12) on the steroid core of cholic acid, and the hydroxyl group at the end of the side chain is combined with glycine through a peptide bond. [0003] CG is synthesized by liver cells, and its normal metabolic pathway is enterohepatic circulation. It is discharged into the gallbladder through the capillary and bile ducts, and enters the duodenum along with bile to help digest food. 95% of bile acid is reabsorbed in the terminal ileum, and then returned to the liver thr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/546
CPCG01N33/546
Inventor 李元丽
Owner CHONGQING ZHONGYUAN BIOLOGICAL TECH
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