RNA polymerase transcription promoter and nucleic acid sequencing method

a technology of transcription promoter and nucleic acid, applied in the direction of transferases, organic chemistry, biochemistry apparatus and processes, etc., can solve the problems of et al having some problems, affecting the synthesis of rna or nucleic acid, and the evaluation method of a transcription promoting ability
US20030073202A1Inactive Publication Date: 2003-04-17IWATA MASAAKI +1
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Patent Information

Authority / Receiving Office
US · United States
Current Assignee / Owner
IWATA MASAAKI
Publication Date
2003-04-17
Estimated Expiration
Not applicable · inactive patent

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Abstract

An RNA polymerase transcription accelerator comprising a compound represented by the following Formula (I) or salts thereof. A method of sequencing DNA in which nucleic acid transcripts are obtained using an RNA polymerase and a DNA fragment as a template, the resulted nucleic acid transcripts are separated, the nucleic acid sequence is determined from the separated fractions wherein the nucleic acid transcription reaction is carried out in the presence of a compound selected from a group of compounds represented by the above formula (I). The polyamine compounds above have outstanding accelerating activity on transcription activity of RNA polymerase. Therefore, use of the polyamine compounds in a DNA sequencing method using RNA polymerase can make a length of DNA sequence that can be determined in one sequencing longer.
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Description

[0001] The present invention relates to polyamine compounds which accelerate the transcription reaction of RNA polymerase (hereinafter referred to as RNAP). Further, the present invention relates to a DNA sequencing method using RNAP with the polyamine compounds and an initiator of nucleic acid transcription reaction by RNAP.BACKGROUND TECHNOLOGY

[0002] A DNA sequencing method is one of the most important means in molecular biological field. One of the most useful nucleic acid sequencing method at present is a direct transcription sequencing method (W096 / 14434) using RNAP such as T7 RNAP and a terminator of RNA transcription reaction (for example, 3'-deoxyribonucleotide-5'-triphospha-te, 3'dNTPs). This method is an outstanding method utilizing the RNAP transcription reaction for sequencing nucleic acid sequences of DNA products amplified by polymerase chain reaction without removing primers and 2'-deoxyribonucleoside-5-triphosphates (2'dNTPs). Recently, it has been suggested that the...

Claims

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