Application of cationized polysaccharide in preparation of medicines for bacterial biomembrane control

A cationization and biofilm technology, applied in the field of biomedicine, can solve the problems of ineffective drug treatment, etc., and achieve the effects of good compatibility with organisms, better drug efficacy, and high raw material selectivity

Inactive Publication Date: 2018-12-07
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the control of bacterial biofilm is mainly through the use of antibiotics, but the bacteria that eventually form biofilms ar

Method used

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  • Application of cationized polysaccharide in preparation of medicines for bacterial biomembrane control
  • Application of cationized polysaccharide in preparation of medicines for bacterial biomembrane control
  • Application of cationized polysaccharide in preparation of medicines for bacterial biomembrane control

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Preparation of C-Dextran solution.

[0049] (1) Preparation of C-Dextran

[0050] Weigh a certain amount of dextran in DMSO, heat to 45°C to dissolve for 3 hours, cool to room temperature, add excess carbonyldiimidazole (CDI), react at room temperature for 3 hours to fully activate, then add excess ethylenediamine, continue stirring for 18 hours , to make it fully reacted, dialyzed for three days, and freeze-dried for later use;

[0051] Wherein, the molar ratio of dextran and DMSO is 5:10 6 , the mass ratio of dextran to carbonyldiimidazole is 1:2, and the molar ratio of dextran to ethylenediamine is 1:10 5 ;

[0052] (2) Preparation of C-Dextran solution

[0053] Weigh a certain amount of C-Dextran, add a certain amount of 4 ~ 18MΩ.cm pure water to prepare a 100mg / ml mother solution, filter and sterilize through a 0.22μm filter and store at -20°.

Embodiment 2

[0055] Preparation of C-BSP solution

[0056] (1) Preparation of C-BSP

[0057] Weigh a certain amount of Bletilla striata polysaccharide in DMSO, heat at 45°C to dissolve for 3 hours, cool to room temperature, add excess carbonyldiimidazole (CDI), react at room temperature for 3 hours to fully activate, then add excess ethylenediamine, and continue stirring for 18 hours to make It was fully reacted, dialyzed for three days, and freeze-dried for later use;

[0058] The mol ratio of above-mentioned bletilla striata polysaccharide and DMSO is 5:10 6 , the mass ratio of Bletilla striata polysaccharide to carbonyldiimidazole is 1:2, and the molar ratio of Bletilla striata polysaccharide to ethylenediamine is 1:10 5 ;

[0059] (2) Preparation of C-BSP mother liquor

[0060] Weigh a certain amount of C-BSP, add a certain amount of 4 ~ 18MΩ.cm pure water to prepare a 100mg / ml mother liquor, filter and sterilize with a 0.22um filter and store in -20°C.

Embodiment 3

[0062] Preparation of C-KGM solution

[0063] (1) Preparation of C-KGM

[0064] Weigh a certain amount of konjac polysaccharide in DMSO, heat at 45°C to dissolve for 3 hours, cool to room temperature, add excess carbonyldiimidazole (CDI), react at room temperature for 3 hours to fully activate, then add excess ethylenediamine, and continue stirring for 18 hours to make It was fully reacted, dialyzed for three days, and freeze-dried for later use;

[0065] The molar ratio of konjac polysaccharide and DMSO is 5:10 6 , the mass ratio of bletilla striata polysaccharide to carbonyldiimidazole is 1:2, and the molar ratio of konjac polysaccharide to ethylenediamine is 1:10 5 ;

[0066] (2) Preparation of C-KGM mother liquor

[0067] Weigh a certain amount of C-KGM, add a certain amount of 4 ~ 18MΩ.cm pure water to prepare a 100mg / ml mother liquor, filter and sterilize with a 0.22um filter and store in -20°C.

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Abstract

The invention discloses an application of a cationized polysaccharide, particularly an application of a cationized polysaccharide in preparation of antibacterial biomembrane agents, antibacterial coating/spraying agents for biological medical devices, and antibacterial functional materials. The cationized polysaccharide is a polysaccharide having positive charges and is prepared by reacting a natural polysaccharide with a quaternary ammonium salt or a polyamine compound. The cationized polysaccharide can interact with negative-charge polysaccharides in bacteria and biomembrane extracellular matrixes, has excellent sterilization effects, and can be applied well in chronic inflammation diseases, devices for external use or device surface antibiosis, and antibacterial biological materials such as daily chemical products, package products and household paper containing cationized polysaccharides.

Description

technical field [0001] The invention relates to the application of a cationized polysaccharide, in particular to the use of a cationized polysaccharide in the preparation of reagents or functional materials for preventing and controlling bacterial biofilms, and belongs to the technical field of biomedicine. Background technique [0002] Polysaccharides of natural polymers have good biocompatibility and biological activity, and are widely used in clinical and biomedical fields. At present, more studies are made on semi-synthetic cationic modified natural polysaccharides, such as cationic chitosan (C-Dextran), cationic bletilla striata polysaccharide (C-BSP) or cationic konjac polysaccharide (C-KGM). These polysaccharides are densely positively charged due to the protonation of the modified amino groups in aqueous solution. This feature allows cationic polysaccharides to tightly bind negatively charged nucleic acids and serve as carriers for nucleic acid drug delivery. In the...

Claims

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Application Information

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IPC IPC(8): A61K31/724A61K31/716A61K31/715A01N43/16A01P1/00A61P31/04A61P31/02A61P17/02A61P29/00
CPCA01N43/16A61K31/715A61K31/716A61K31/724A61P17/02A61P29/00A61P31/02A61P31/04A61K2300/00
Inventor 董磊李玉荣甘璟璟
Owner NANJING UNIV
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